Hormone peptides PYY and TFF2 action on leptin signaling pathway in CLU189 cells

• Grant number: 15/03645-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2015
• End date: March 31, 2016
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Licio Augusto Velloso
• Grantee: Rafael Marostica
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 13/07607-8 - OCRC - Obesity and Comorbidities Research Center, AP.CEPID

Abstract

The control of food intake involves several mechanisms regulated by interactions between intestine, central nervous system (CNS) and the adipose tissue. These organs, when stimulated, produce hormones related to energetic homeostasis, like insulin and leptin. Beyond the hypothalamic action of insulin and leptin on the regulation of energetic homeostasis, it is known, nowadays, that some gastrointestinal peptides also have an importance role in this system. Thus, a fine interplay between the CNS, gastrointestinal system and adipose tissue is necessary. The intestinal peptides Y3-36 (PYY3-36) and Trefoil Factor Family 2 (TFF2) are examples of gastrointestinal peptides which induce satiety. PYY3-36 and TFF2 modulates the activity of some neuronal populations in the arcuate nucleus, like NPY/AgRP and POMC/CART, reducing food intake and increasing energetic expenditure. Thus, the aim of this project is to investigate the PYY3-36 and TFF2 action on leptin signaling in hypothalamic cells of mice. For this, we will use a hypothalamic adult cell line (mHypoA-2/29 CLU189) to investigate leptin, palmitic acids or specific intestinal peptides challenge and the impact on the regulation of food intake. By Western blotting and RT-PCR, we will analyze the expression of PYY and TFF2 receptors as well as NPY/AgRP and POMC/CART neuropeptides. We believe that this project will help clarify the mechanisms regulated by gastrointestinal peptides in the control of food intake and energy homeostasis and will produce groundbreaking knowledge to scientific community.