Identification of the bacteria associated with bovine periodontitis and oral health using bacterial culture and high-throughput 16S rRNA gene sequencing

• Grant number: 15/06917-9
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: July 20, 2015
• End date: December 19, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Iveraldo dos Santos Dutra
• Grantee: Ana Carolina Borsanelli
• Supervisor: Marcello Pasquale Riggio
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Institution abroad: University of Glasgow, Scotland
• Associated to the scholarship: 13/13701-7 - Genotyping of anaerobic bacteria isolated from bovine periodontitis lesions, BP.DR

Abstract

There is little known about periodontal disease in cattle and no routine treatments and prevention currently exist. Although cattle are of worldwide economic importance in the dairy and beef industries, their dentition has not been investigated as thoroughly as that in other herbivores and small animals. Bovine periodontitis is one such disease of cattle whose importance has been overlooked by veterinarians and scientists for many decades. "Cara inchada" in cattle is a purulent progressive periodontitis associated with strict anaerobic Gram-negative microorganisms, mainly from the Bacteroides genus, which forming black-pigmented colonies in culture medium with hemin and vitamin K. It has been estimated that approximately 50% of the human oral microflora is uncultivable. Previously uncharacterized and uncultivable bacteria have been identified in human periodontitis. A significant proportion of bacteria in the bovine oral cavity may also be uncultivable but nevertheless contribute to the disease process. The strength of culture-independent methods is that as well as detecting cultivable bacteria they can also be used to identify bacteria that are uncultivable or very fastidious in their growth requirements and, in addition, identify novel species. Therefore, culture-independent methods can be used to identify uncultivable bacteria and even novel species. In the proposed study we intend to use high-throughput bacterial 16S rRNA gene sequencing to determine the composition of the complex microbial communities associated with bovine periodontitis and oral health. Tradicional microbiological culture methods will also be used to isolate and identify bacteria from clinical specimens. This study will provide the most detailed insight to date into the composition of the bovine oral microbiome associated with oral health and periodontitis and is the first step in elucidating the true microbial nature of the disease. The work is original and of great significance to the national economy, to bovine health and represents a major evolution for scientific knowledge. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BORSANELLI, ANA C.; LAPPIN, DAVID F.; VIORA, LORENZO; KING, GEORGE; BENNETT, DAVID; DUTRA, IVERALDO S.; RIGGIO, MARCELLO P.. Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health. Research in Veterinary Science, v. 118, p. 439-443, JUN 2018. (15/06917-9)

BORSANELLI, ANA C.; LAPPINB, DAVID F.; VIORA, LORENZO; BENNETT, DAVID; DUTRA, IVERALDO S.; BRANDT, BERND W.; RIGGIO, MARCELLO P.. Microbiomes associated with bovine periodontitis and oral health. Veterinary Microbiology, v. 218, p. 1-6, MAY 2018. (15/06917-9)

BORSANELLI, A. C.; VIORA, L.; PARKIN, T.; LAPPIN, D. F.; BENNETT, D.; KING, G.; DUTRA, I. S.; RIGGIO, M. P.. Risk factors for bovine periodontal disease - a preliminary study. ANIMAL, v. 15, n. 2, FEB 2021. (15/06917-9)