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Study of tracheobronchial lavage of calves using metagenomics

Grant number: 15/02947-0
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): August 10, 2015
Effective date (End): February 09, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal researcher:Lilian Gregory
Grantee:Natália Carrillo Gaeta
Supervisor abroad: Rodrigo Carvalho Bicalho
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Research place: Cornell University, United States  
Associated to the scholarship:14/03188-3 - Clinical evaluation of bronchopneumonia of calves in the settlements in the region of Presidente Epitácio and Presidente Venceslau, BP.MS


A quest for bacterial communities present in different environments became an important goal within the scientific community, in order to know the role of each one within an environment. The term "metagenomics" was first used in 1998 by Jo Handelsman, from University of Wisconsin - USA and refers to a culture-independent study of a collection of genome of a diverse of bacterial communities that can be used in exploration of all bacterial genomes that reside in the environment, plants and animals. Because of the clinical importance of this new technology that identifies all bacterial genera in a sample, including those whose cultive is difficult by standard techniques, the aim of this study is evaluate tracheobronquical wash microbiome using metagenomics. This study will be conducted in the Department of Population Medicine and Diagnostic Science da Veterinary College, University of Cornell, New York, EUA.DNA from all samples will be extracted using QIAamp DNA minikit (Qiagen) and the sequencing of the 16S rRNA gene will be performed using Illumina GAIIx platform. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GAETA, NATALIA C.; LIMA, SVETLANA F.; TEIXEIRA, ANDRE G.; GANDA, ERIKA K.; OIKONOMOU, GEORGIOS; GREGORY, LILIAN; BICALHO, RODRIGO C. Deciphering upper respiratory tract microbiota complexity in healthy calves and calves that develop respiratory disease using shotgun metagenomics. JOURNAL OF DAIRY SCIENCE, v. 100, n. 2, p. 1445-1458, FEB 2017. Web of Science Citations: 10.

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