In vitro analysis of an active ingredient for control of microorganisms of interest and dental assessment of cytotoxicity and genotoxicity

• Grant number: 15/05561-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2015
• End date: July 31, 2015
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Samira Esteves Afonso Camargo
• Grantee: Denise Verônica Pena
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

This study aims to evaluate the antimicrobial activity of the active ingredient thymol of Equisetum arvense L. (horsetail) on Candida albicans, Staphylococcus aureus and Streptococcus mutans and also their cytotoxicity and genotoxicity in cultured macrophages (RAW 264.7). After determining the Microbicide Low Concentration (CMM) using the broth microdilution extract their cytotoxicity is evaluated by MTT method. Further, genotoxicity is determined by the formation of micronuclei in cells after 24 h of exposure to the active ingredient and sulfanato etilmetano (positive control). The data will be evaluated by ANOVA and Tukey's multiple comparison test, both adopting a significance level of 5%.