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STRUCTURAL CHARACTERIZATION OF THE ACESSORY GLANDS AND NEUROENDOCRINE COMPONENTS OF THE DIGESTIVE SYSTEM OF PUFFERFISH Sphoeroides testudineus (LINNAEUS 1758) (Tetraodontidae: Tetraodontiformes)

Grant number: 15/02084-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2015
Effective date (End): November 30, 2016
Field of knowledge:Biological Sciences - Morphology - Histology
Principal Investigator:Renata de Britto Mari
Grantee:Kainã Rocha Cabrera Fagundes
Home Institution: Instituto de Biociências (IB-CLP). Universidade Estadual Paulista (UNESP). Campus Experimental do Litoral Paulista. São Vicente , SP, Brazil

Abstract

Fish have a great biodiversity of species and inhabit a range of environments. The morphology allows a better understanding of the ecology and feeding habits of the species.Changes in the morphology of the digestive system of fish are reported in some species, such as puffer fish, belonging to the order Tetraodontiformes, have specialization of the digestive system that allows you to expand the body through ingestion of water or air as a defense against predators.The puffer fish Sphoeroides testudineus (LINNEAUS 1758) feeds mainly on bivalve molluscs, gastropods and macroalgae and can be found in the Estuarine System of Santos and São Vicente. In previous studies, it was concluded that this species has a flared portion of the esophagus which is responsible for body expansion and chemical digestion of food. Another remark is the absence of the pancreas and the presence of a prominent liver, occupying more than one-third of the body. Fish can present the exocrine portion of the pancreas dispersed in the liver and mesentery.The endocrine portion can be in the gastroenteropancreatic system form (or enteropancreatic) and the cells may be classified as enterochromaffin or not and closed and open-ended system.The somatostatin-producing cells are not enterochromaffin and may be highlighted by marking the chemical messenger by immunohistochemistry.This messenger is of great importance in the regulation of insulin and glucagon in the body.Therefore, this study aims to describe the morphology of the accessory glands, highlight the argirophilly neuroendocrine components of the Enteropancreatic System and the immunoreactive cells to somatostatin to evidence the pancreas in S. testudineus. For this, will be used 30 adult specimens of S. testudineus coming from artisanal fisheries in the Bay of Santos, which it will be collected portions of the proximal, middle and distal intestine, and then subjected to techniques as HE for a general description of the accessory glands; Grimelius for viewing the argirophilly neuroendocrine components of the Enteropancreatic System and immunohistochemistry with a specific antibody for somatostatin.