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Development and validation of an analytical method by hydrophilic interaction chromatography: HILIC friendly to the environment for analyses to Ertapenem sodium in lyophilized powder for injectable solution

Grant number: 15/03412-3
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): September 01, 2015
Effective date (End): August 31, 2016
Field of knowledge:Health Sciences - Pharmacy - Medicines Analysis and Control
Principal Investigator:Hérida Regina Nunes Salgado
Grantee:Tahisa Marcela Pedroso
Supervisor abroad: Frédéric Denise Antoine Lynen
Home Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Local de pesquisa : Ghent University (UGent), Belgium  
Associated to the scholarship:13/12959-0 - Development and validation of analytical methods for characterization and quantification of Ertapenem sodium in lyophilized powder for injectable solution, BP.DR


Antimicrobials have unquestionable importance in the control of many diseases; however the constant concern with evolution of resistant microorganisms is increasing. Ertapenem sodium is a beta-lactam antibiotic of the carbapenem class that has a broader activity spectrum than most other ²-lactam antimicrobials and is more resistant to the enzyme ²-lactamase, which is the main mechanism of resistance of many bacteria. The progress of microbial resistance to existing antibiotics is alarming. Thus we need to preserve antimicrobials that still have activity against these pathogens. In this context, quality control has a key role to ensure the correct dosage, by contributing preventively to minimize the development of resistant microorganisms. Study of the physicochemical characteristics of the drug and quantification of the content of the active substance are of fundamental importance for the pharmaceutical industry to ensure the quality of the product sold. Currently, pharmacists have concentrated their focus and creative energies toward minimizing the environmental impact of their craft. Ertapenem is the polar and ionizable compound and therefore it has little retention on reversed phase LC, even using highly aqueous mobile phase, which is worrying because traditional C18 columns can result dewetting in this condition. Thus develop a method for Hydrophilic Interaction Chromatography - HILIC is the most suitable for this drug. But traditional methods in HILIC are necessary the use of highly organic mobile phase, usually an amount exceeding 80% acetonitrile is necessary. Develop a method for HILIC that is friendly to the environment in the area of green chemistry is challenging, but it is fully possible. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEDROSO, TAHISA M.; MEDEIROS, ANA C. D.; SALGADO, HERIDA R. N. RP-HPLC x HILIC chromatography for quantifying ertapenem sodium with a look at green chemistry. Talanta, v. 160, p. 745-753, NOV 1 2016. Web of Science Citations: 8.

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