Development of hydrogels based on hyaluronic acid/collagen/chitosan for bone bioengineering

• Grant number: 15/09777-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2015
• End date: November 30, 2015
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Katiúcia Batista da Silva Paiva
• Grantee: Diego Garcia Miranda
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 10/08918-9 - Gene-modified-tissue engineering of bone: overexpression and inhibition of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs and RECK) by osteoblasts from human mesenchymal stem cells seeded on collagen-chitosan, AP.JP

Abstract

The increase in life expectancy of the population is associated with high costs of medical treatments, since the population is more susceptible to degenerative and chronic diseases and accidents. The spending on orthopedic treatments, including prosthetics and orthotics, have increased gradually and became a public health problem, mainly due to car accidents. Thus, the social cost reduction caused by traumatic and degenerative diseases of the musculoskeletal system and mineralized tissues, is increasingly dependent on the development of novel therapies which can promote bone regeneration. In this context, Tissue Bioengineering, where cells are associated with biomaterials and bioactive molecules for tissue regeneration , it has become a new possibility.. Thus, the scientific community has invested heavily in search of better sources of stem cells and the development of new biomaterials, seeking new therapeutic approaches within the Bone Bioengineering. Our group has been researching alternative sources of stem cells with osteogenic potential as well as new biomaterials. Given the above, this project aims to develop hydrogels of hyaluronic acid base/collagen/chitosan cross linked chemically and assess their osteogenic potential in vitro from stem cells from human dental pulp (DPSCs).