Development of a carrier-based poloxamer system incorporated with beta-tricalcium phosphate doped with magnesium and/or zinc and teicoplanin aiming the osteomyelitis treatment associated with bone repair

• Grant number: 13/11534-6
• Support Opportunities: Regular Research Grants
• Duration: October 01, 2014 - September 30, 2016
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Juliana Marchi
• Grantee: Juliana Marchi
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated researchers: Daniele Ribeiro de Araujo ; Fabiano Yokaichiya ; José Fabián Schneider ; Leila Soares Ferreira ; Luis Gallego Martinez ; Marcelo Lancellotti

Abstract

Osteomyelitis is a bacterial bone infection common in many traumas. The conventional treatment of such chronic disease is the debridement of infected bone and adjacent soft tissue, associated with antibiotic treatment. This work proposes the development of an injectable carrier-based poloxamer incorporated with teicoplanin (as antibiotic) and different beta-tricalcium phosphate (beta-TCP) compositions doped with magnesium and/or zinc for treatment of osteomyelitis associated with bone repair. Poloxamer 407 (PL407) and 188 (PL188) are thermoreversible hydrogels whose gelation occurs near body temperature (37°C) and at low temperatures shows as a liquid form. These properties associated with their biocompatibility and biodegradability make PL407 and PL188 extensively studied as a vehicle for drug delivery, so that they can be injected in liquid form and undergo gelation in situ, without the disadvantages presented by polymethyl methacrylate. The beta-TCP ceramics were synthesized and physically, chemically and biologically characterized in a previous project (FAPESP no. 2009/10745-8). From the results obtained, the materials with the best behavior have been chosen for the incorporation into hydrogel predicted in this project. The characterization of the materials will be performed by determining the solubility and partition coefficient micelle-drug, the sol-gel transition temperature and the temperature of micellization, as well as permeation study and drug release kinetics in vitro. The biological characterization of in vitro tests will be carried out through bacterial susceptibility, indirect cytotoxicity, cell migration and osteogenic cell differentiation. (AU)

Filed patent(s) as a result of this research project