Microbiological analysis on a new anatomical pieces preparing technique for use on veterinary surgery

• Grant number: 15/08826-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2015
• End date: May 31, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Fabrício Singaretti de Oliveira
• Grantee: Natália Pereira
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

In order to avoid tissue deterioration, anatomical pieces are fixed. Fixation technique is extremely important, since it keeps tissues strong, insoluble and protected. Formaldehyde is the most used fixing material and preservative, and, although it is still very useful at anatomy labs, it's hazardous and also brings lots of environmental risks. Other agents, as ethanol, have been used as good fixing agents for birds muscle, as has the Sodium chloride been testes as preservative for anatomical pieces. In this way, this research aims to identify and to quantify, microbiologically, the main species on fixation of dog corpses by using ethanol and on conservation on 30% sodium chloride aqueous solution. Thus, fixation/conservation solutions will be tested for microbiology by quantifying aerobic mesophilic and facultatively anaerobic bacteria and performed the counting of colony forming unit for further Bacillus, Clostridium, and Pseudomonas genera, and Escherichia coli species identification. First results show a great potential in using these substances for fixing and keeping the anatomical pieces for four months, in a way that they keep flexibility and viscera aspect similar to a fresh corpse.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEREIRA, NATALIA; CARDOZO, MARITA VEDOVELLI; DE SA ROCHA, THIAGO ANDRE SALVITTI; ZERO, RAPHAEL CHIARELO; DE AVILA, FERNANDO ANTONIO; DE OLIVEIRA, FABRICIO SINGARETTI. Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution. SEMINA-CIENCIAS AGRARIAS, v. 40, n. 6, 2, p. 3099-3105, NOV-DEC 2019. (15/08259-9, 15/08826-0)

PEREIRA, NATALIA; CARDOZO, MARITA VEDOVELLI; DE SA ROCHA, THIAGO ANDRE SALVITTI; ZERO, RAPHAEL CHIARELO; DE AVILA, FERNANDO ANTONIO; DE OLIVEIRA, FABRICIO SINGARETTI. Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution. SEMINA-CIENCIAS AGRARIAS, v. 40, n. 6, p. 7-pg., 2019-11-01. (15/08826-0, 15/08259-9)