Preparation, evaluation and separation optimization of porous-layer open-tubular (PLOT)columns for nano-LC-MS/MS analysis of sulfonamides and their acetylated metabolites in food samples

• Grant number: 15/12306-2
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: September 30, 2015
• End date: September 29, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Fernando Mauro Lanças
• Grantee: Meire Ribeiro da Silva
• Supervisor: Elsa Lundanes
• Host Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Institution abroad: University of Oslo (UiO), Norway
• Associated to the scholarship: 12/22055-9 - Development of silica modified with ionic liquids and their use in sample preparation techniques, BP.DR

Abstract

The presence of antibiotic residues such as sulfonamides in milk interferes with the manufacture of some dairy products, may cause allergic reactions in humans, as well as antibiotic resistance. The antibiotic metabolites residues can be even more hazardous and more persistent than the original compounds. Along the years the definition of ''residue'' of many contaminants have been modified and some regulatory agencies have been included the contaminant metabolites in their maximum residue limits (LMR). In this context residue monitoring of antibiotics and their metabolites in food sample is an important activity to guarantee the safety of food. In order to monitoring sulfonamides and their acetylated metabolites in milk samples to get separation efficiency and lower limit of the detection (LOD), studies will be undertaken employing high efficiency porous layer open tubular (PLOT) columns and also separation techniques coupled to tandem mass spectrometry in a nano -LC (PLOT) - MS/MS set up. After the analytical method development and validation the system will be employed in the analysis of sulfonamides in real food samples. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, MEIRE RIBEIRO; LANCAS, FERNANDO MAURO. Evaluation of ionic liquids supported on silica as a sorbent for fully automated online solid-phase extraction with LC-MS determination of sulfonamides in bovine milk samples. JOURNAL OF SEPARATION SCIENCE, v. 41, n. 10, p. 2237-2244, MAY 2018. (12/22055-9, 15/12306-2)

DOMINGUES NAZARIO, CARLOS EDUARDO; FUMES, BRUNO HENRIQUE; DA SILVA, MEIRE RIBEIRO; LANCAS, FERNANDO MAURO. New materials for sample preparation techniques in bioanalysis. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1043, p. 81-95, FEB 1 2017. (12/22055-9, 15/15462-5, 14/07347-9, 15/12306-2)

DA SILVA, MEIRE RIBEIRO; BRANDTZAEG, OLE KRISTIAN; VEHUS, TORE; LANCAS, FERNANDO MAURO; WILSON, STEVEN RAY; LUNDANES, ELSA. An automated and self-cleaning nano liquid chromatography mass spectrometry platform featuring an open tubular multi-hole crystal fiber solid phase extraction column and an open tubular separation column. Journal of Chromatography A, v. 1518, p. 104-110, OCT 6 2017. (15/12306-2)