Global signal transduction involved in the immediate response to the two surfaces of titanium alloys: building OsteoBLAST database

• Grant number: 15/03639-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2015
• End date: February 28, 2017
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Willian Fernando Zambuzzi
• Grantee: Marcel Rodrigues Ferreira
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Current trends in implant therapy have included the modification of their surfaces using nanotechnology tools and principles of bioengineering, enhancing their performance. Although much has been achieved in tools for developing these materials, biological analysis and methodologies did not advance at this speed. Based on current needs on novel tools able to help the most orderly development of biomaterials, the main objective of this project is to evaluate the effect of different surfaces of biomedical materials on osteoprogenitor cell response, especially as regards: signals transduction mechanisms and morphological changes with a view to building a Database able to assist the production of novel biomaterials - to this platform, we will call: OsteoBLAST. Through collaboration with the group led by Prof. Maikel Peppelenbosch, we will use the microarray peptides (Pepchip) to monitor global signal transduction mechanisms involved in the response to different materials, establishing a collection of biological data. In addition, we will also evaluate the secretome of these cells by proteomic analysis. Subsequently, these data will feed the OsteoBLAST database. In conclusion, we expect the product generated at the end of this project serve as a benchmark for further analysis, focusing its diffusion in the productive sector in the biomaterials area. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, MARCEL R.; SANTOS, GERSON A.; BIAGI, CARLOS A.; SILVA JUNIOR, WILSON A.; ZAMBUZZI, WILLIAN F.. GSVAscore reveals molecular signatures from transcriptomes for biomaterials comparison. Journal of Biomedical Materials Research Part A, v. 109, n. 6, SEP 2020. (14/22689-3, 18/05731-7, 15/03639-8, 13/08135-2)

DA COSTA FERNANDES, CELIO J.; FERREIRA, MARCEL RODRIGUES; BEZERRA, FABIO J. B.; ZAMBUZZI, WILLIAN F.. Zirconia stimulates ECM-remodeling as a prerequisite to pre-osteoblast adhesion/proliferation by possible interference with cellular anchorage. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, v. 29, n. 4, APR 2018. (15/03639-8, 14/22689-3, 16/08888-9)

FERREIRA, MARCEL RODRIGUES; MILANI, RENATO; RANGEL, ELIDIANE C.; PEPPELENBOSCH, MAIKEL; ZAMBUZZI, WILLIAN. OsteoBLAST: Computational Routine of Global Molecular Analysis Applied to Biomaterials Development. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 8, OCT 8 2020. (18/05731-7, 14/22689-3, 15/03639-8)

BEZERRA, FABIO; FERREIRA, MARCEL R.; FONTES, GISELLE N.; DA COSTA FERNANDES, CELIO JR; ANDIA, DENISE C.; CRUZ, NILSON C.; DA SILVA, RODRIGO A.; ZAMBUZZI, WILLIAN F.. Nano hydroxyapatite-blasted titanium surface affects pre-osteoblast morphology by modulating critical intracellular pathways. Biotechnology and Bioengineering, v. 114, n. 8, p. 1888-1898, AUG 2017. (15/03639-8, 14/22689-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FERREIRA, Marcel Rodrigues. OsteoBLAST: computational routine of global molecular analysis combined with systemic biology and applied to the production of biomaterials. 2017. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.