Vaccines for control of dengue: vaccine potential of the combination of the non-structural proteins in the generation of protective cell response in an experimental model

• Grant number: 15/02352-7
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): August 01, 2015
• Effective date (End): October 07, 2020
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal researcher: Luis Carlos de Souza Ferreira
• Grantee: Rubens Prince dos Santos Alves
• Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 17/25166-0 - Development of an immunocompetent animal model for the study of dengue vaccines in the context of DENV-exposed and naïve groups, BE.EP.DR

Abstract

Dengue fever, a disease caused by one of four serotypes of dengue virus (DENV 1-4), which is the main arbovirus currently affects humans. Although the disease is known for over 70 years, there are no effective treatments for the disease, as well as vaccine formulations available and capable of conferring protection against the infection. Clinical tests studies indicate that induction of high titers of neutralizing antibodies is not a suitable protective correlate therefore a more detailed review of the role of cellular immunity in protection against dengue becomes particularly relevant. Thus, this project aims to explore the role of non-structural proteins of dengue virus type 2 (DENV2) in immune response induction protective cell in an experimental model. For this purpose vaccine formulations composed of three major non-structural proteins: NS1, NS3 and NS5 as recombinant proteins and DNA vaccines used in vaccine compositions for immunization in an animal model. Adaptive immune response profiles are weighed against the different protocols immunization, ensuring that the cellular profile found may be associated or not with viral protection. The knowledge generated from the research proposal will reassess the current correlates involved in protection against infection by DENV, as well as facilitate the development of effective vaccines against the disease. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARAUJO, SERGIO C.; PEREIRA, LENNON R.; ALVES, RUBENS P. S.; ANDREATA-SANTOS, ROBERT; KANNO, ALEX I.; FERREIRA, LUIS CARLOS S.; GONCALVES, VIVIANE M.. Anti-Flavivirus Vaccines: Review of the Present Situation and Perspectives of Subunit Vaccines Produced in Escherichia coli. VACCINES, v. 8, n. 3, SEP 2020. (18/24683-3, 16/20045-7, 16/23560-0, 19/18205-4, 15/02352-7)

DOS SANTOS ALVES, RBENS PRINCE; PEREIRA, LENNON RAMOS; NASCIMENTO FABRIS, DENICAR LINA; SALVADOR, FELIPE SCASSI; SANTOS, ROBERT ANDREATA; DE ANDRADE ZANOTTO, PAOLO MARINHO; ROMANO, CAMILA MALTA; AMORIM, JAIME HENRIQUE; DE SOUZA FERREIRAA, LUIS CARLOS. Production of a Recombinant Dengue Virus 2 NS5 Protein and Potential Use as a Vaccine Antigen. Clinical and Vaccine Immunology, v. 23, n. 6, p. 460-469, JUN 2016. (14/17595-0, 15/02352-7, 12/21077-9)

ANDREATA-SANTOS, ROBERT; DOS SANTOS ALVES, RUBENS PRINCE; PEREIRA, SARA ARAUJO; PEREIRA, LENNON RAMOS; DE FREITAS, CARLA LONGO; PEREIRA, SAMUEL SANTOS; VENCESLAU-CARVALHO, ALEXIA ADRIANNE; CASTRO-AMARANTE, MARIA FERNANDA; PINHO FAVARO, MARIANNA TEIXEIRA; MATHIAS-SANTOS, CAMILA; et al. Transcutaneous Administration of Dengue Vaccines. Viruses-Basel, v. 12, n. 5, MAY 2020. (16/05570-8, 12/50362-3, 16/20045-7, 14/17595-0, 16/23560-0, 15/02352-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALVES, Rubens Prince dos Santos. Dengue Vaccine: Potential of Combining Non-structural Proteins in Induce Protective Cell Response in an Experimental Model. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.