Vaccines for control of dengue: vaccine potential of the combination of the non-structural proteins in the generation of protective cell response in an experimental model

• Grant number: 15/02352-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2015
• End date: October 07, 2020
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Luis Carlos de Souza Ferreira
• Grantee: Rúbens Prince dos Santos Alves
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 17/25166-0 - Development of an immunocompetent animal model for the study of dengue vaccines in the context of DENV-exposed and naïve groups, BE.EP.DR

Abstract

Dengue fever, a disease caused by one of four serotypes of dengue virus (DENV 1-4), which is the main arbovirus currently affects humans. Although the disease is known for over 70 years, there are no effective treatments for the disease, as well as vaccine formulations available and capable of conferring protection against the infection. Clinical tests studies indicate that induction of high titers of neutralizing antibodies is not a suitable protective correlate therefore a more detailed review of the role of cellular immunity in protection against dengue becomes particularly relevant. Thus, this project aims to explore the role of non-structural proteins of dengue virus type 2 (DENV2) in immune response induction protective cell in an experimental model. For this purpose vaccine formulations composed of three major non-structural proteins: NS1, NS3 and NS5 as recombinant proteins and DNA vaccines used in vaccine compositions for immunization in an animal model. Adaptive immune response profiles are weighed against the different protocols immunization, ensuring that the cellular profile found may be associated or not with viral protection. The knowledge generated from the research proposal will reassess the current correlates involved in protection against infection by DENV, as well as facilitate the development of effective vaccines against the disease. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANDREATA-SANTOS, ROBERT; MACHADO, RAFAEL RAHAL GUARAGNA; DOS SANTOS ALVES, RUBENS PRINCE; SALES, NATIELY SILVA; SOARES, CAMILA PEREIRA; RODRIGUES, KARINE BITENCOURT; SILVA, MARIANGELA OLIVEIRA; FAVARO, MARIANNA TEIXEIRA DE PINHO; RODRIGUES-JESUS, MONICA JOSIANE; YAMAMOTO, MARCIO MASSAO; et al. Validation of Serological Methods for COVID-19 and Retrospective Screening of Health Employees and Visitors to the Sao Paulo University Hospital, Brazil. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 12, p. 8-pg., 2022-06-02. (20/08943-5, 16/20045-7, 21/05661-1, 14/50890-5, 16/23560-0, 15/02352-7, 20/06409-1, 17/24769-2, 16/14344-1, 18/23680-0, 20/10700-3, 18/07629-5, 18/07142-9)

DOS SANTOS ALVES, RBENS PRINCE; PEREIRA, LENNON RAMOS; NASCIMENTO FABRIS, DENICAR LINA; SALVADOR, FELIPE SCASSI; SANTOS, ROBERT ANDREATA; DE ANDRADE ZANOTTO, PAOLO MARINHO; ROMANO, CAMILA MALTA; AMORIM, JAIME HENRIQUE; DE SOUZA FERREIRAA, LUIS CARLOS. Production of a Recombinant Dengue Virus 2 NS5 Protein and Potential Use as a Vaccine Antigen. Clinical and Vaccine Immunology, v. 23, n. 6, p. 460-469, JUN 2016. (15/02352-7, 12/21077-9, 14/17595-0)

ANDREATA-SANTOS, ROBERT; DOS SANTOS ALVES, RUBENS PRINCE; PEREIRA, SARA ARAUJO; PEREIRA, LENNON RAMOS; DE FREITAS, CARLA LONGO; PEREIRA, SAMUEL SANTOS; VENCESLAU-CARVALHO, ALEXIA ADRIANNE; CASTRO-AMARANTE, MARIA FERNANDA; PINHO FAVARO, MARIANNA TEIXEIRA; MATHIAS-SANTOS, CAMILA; et al. Transcutaneous Administration of Dengue Vaccines. Viruses-Basel, v. 12, n. 5, MAY 2020. (16/05570-8, 12/50362-3, 16/20045-7, 14/17595-0, 16/23560-0, 15/02352-7)

ALVES, RUBENS PRINCE DOS SANTOS; ANDREATA-SANTOS, ROBERT; DE FREITAS, CARLA LONGO; PEREIRA, LENNON RAMOS; FABRIS-MAEDA, DENICAR LINA NASCIMENTO; RODRIGUES-JESUS, MONICA JOSIANE; PEREIRA, SAMUEL SANTOS; CARVALHO, ALEXIA ADRIANNE VENCESLAU BRITO; SALES, NATIELY SILVA; PERON, JEAN PIERRE SCHATZMANN; et al. Protective Immunity to Dengue Virus Induced by DNA Vaccines Encoding Nonstructural Proteins in a Lethal Challenge Immunocompetent Mouse Model. FRONTIERS IN MEDICAL TECHNOLOGY, v. 2, p. 12-pg., 2020-10-30. (17/26170-0, 14/17595-0, 15/02352-7, 12/21077-9)

ARAUJO, SERGIO C.; PEREIRA, LENNON R.; ALVES, RUBENS P. S.; ANDREATA-SANTOS, ROBERT; KANNO, ALEX I.; FERREIRA, LUIS CARLOS S.; GONCALVES, VIVIANE M.. Anti-Flavivirus Vaccines: Review of the Present Situation and Perspectives of Subunit Vaccines Produced in Escherichia coli. VACCINES, v. 8, n. 3, SEP 2020. (18/24683-3, 16/20045-7, 16/23560-0, 19/18205-4, 15/02352-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ALVES, Rúbens Prince dos Santos. Dengue Vaccine: Potential of Combining Non-structural Proteins in Induce Protective Cell Response in an Experimental Model. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.