Use of Dicer muscle-specific knockout mice to evaluate the effect of the global depletion of microRNAs during hypertrophy, atrophy and aging

• Grant number: 14/24327-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: September 01, 2015
• End date: May 31, 2017
• Field of knowledge: Biological Sciences - Biology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Maeli Dal Pai
• Grantee: Ivan José Vechetti Júnior
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 15/19193-9 - The effect of global depletion of microRNAs in skeletal muscle by Dicer knockout, BE.EP.PD

Abstract

microRNAs (miRNAs) regulate gene expression post-transcriptionally, degrading mRNAs or inhibiting its translation. These molecules are processed by Dicer important enzymes that cleave such as pre-miRNAs becoming mature miRNAs. Knowing the importance of these molecules in the control of many cellular mechanisms, such as control of muscle mass, several studies have tried to investigate the actual role of miRNAs in these physiological processes through functional studies (knockout) in vivo and in vitro. However, these molecules act together in various signaling pathways, making complex understanding how miRNAs behave together in muscle adaptation processes. Thus, to better understand the role of miRNAs in controlling muscle mass, the goal of this project is to investigate the effect of global depletion of microRNAs during hypertrophy processes, atrophy and aging using a Knockout model-specific muscle of Dicer. The animals will be divided into the Sham group (n = 10 mice in the vehicle application) and knockout (tamoxifen induction of application for deletion of Dicer) into the following experimental groups: animals subjected to the hypertrophic process (removal of synergist muscles, n = 10) animals undergoing muscular atrophy (suspending members, n = 10) and envelhicidos animals (24-26 months, n = 10). At the end of experiment the animals will be sacrificed, muscles (soleus and plant) removed and subsequently subjected to morphological, immunohistochemical and molecular analysis (RT-qPCR and Western blot). Data will be submitted to appropriate statistical analysis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VECHETTI, JR., IVAN J.; WEN, YUAN; CHAILLOU, THOMAS; MURACH, KEVIN A.; ALIMOV, ALEXANDER P.; FIGUEIREDO, VANDRE C.; DAL-PAI-SILVA, MAELI; MCCARTHY, JOHN J.. Life-long reduction in myomiR expression does not adversely affect skeletal muscle morphology. SCIENTIFIC REPORTS, v. 9, APR 2 2019. (14/24327-1, 15/19193-9)