Super expression of COWP6 protein from Cryptosporidium parvum aiming the production of monoclonal IgG in mice for diagnostic purpose

• Grant number: 15/14315-9
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: September 01, 2015
• End date: January 29, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Marcelo Vasconcelos Meireles
• Grantee: Alex Akira Nakamura
• Supervisor: Fabio Tosini
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Institution abroad: Istituto Superiore di Sanità (ISS), Italy
• Associated to the scholarship: 13/05622-0 - Expression and purification of Cryptosporidium hominis GP60 and COWP6 Cryptosporidium parvum recombinant proteins aiming the production of polyclonal IgY in chicken for diagnostic purpose, BP.PD

Abstract

Cryptosporidium spp. are intracellular protozoan parasites that infect the epithelial cells of digestive and respiratory tracts of a wide variety of vertebrates. This parasite may cause self-limiting diarrheal illness in immunocompetent individuals, but in immunocompromised patients it may be fatal. Cryptosporidium parvum has many animal hosts including humans, and is a major etiological agent of diarrhea in ruminants. The development of diagnosis and control strategies for cryptosporidiosis has become the main challenge of epidemiological studies. Management of sources of the parasite represents the most important means of preventing widespread outbreaks of cryptosporidiosis, and management is dependent on sensitive and specific diagnostic reagents. The Cryptosporidium oocysts wall proteins (COWP) are a group of proteins located on the surface of the oocyst wall and are involved in its structure and resistance of oocysts in environmental conditions; thereby COWP proteins are potential targets for the development of diagnostic methods, and also for isolation and purification of oocysts through immunological techniques. Our goal is to express recombinant COWP6 by cloning, produce monoclonal IgG in mice and testing of the antibodies against COWP6 for binding to C. parvum oocysts. (AU)