Synthesis and characterization of silver nanoparticles applied to immunoglobulins G detection

• Grant number: 15/06847-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2015
• End date: July 31, 2017
• Field of knowledge: Biological Sciences - Immunology - Immunochemistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Fabio de Lima Leite
• Grantee: Akemi Martins Higa
• Host Institution: Centro de Ciências e Tecnologias para a Sustentabilidade (CCTS). Universidade Federal de São Carlos (UFSCAR). Sorocaba , SP, Brazil

Abstract

The aim of this research project is to study the synthesis, functionalization and characterization of silver nanoparticles (AgNPs) applied to the immunoglobulins G (IgGs) detection, in order to survey these nanoparticles' potential as immunonanosensors in immunoassays. The AgNPs are highlighted by their unique photochemical properties that overcome the organic fluorophores' fluorescence properties. It is worth noting that the biological compatibility through the functionalization of the AgNPs' surfaces with biomolecules ensure their application as immunonanosensors able to detect small quantities of molecules in solution. In the biomedical field, the characterization of these photochemical properties and the surface functionalization strategies comprise important studies that contribute to good clinical diagnostic practice. The AgNPs exhibit excellent optical properties that enable their use in specific biomolecules detection assays. Besides the best AgNPs' optical properties, the gold nanoparticles are more widespread in nanobiosensor's studies. The purpose of this project is undergoing an experimental study of the AgNPs to be applied in the immunopathology detection, e.g., Neuromyelitis optica (NMO) and Multiple Sclerosis (MS). The project's auto reactive IgGs will be the anti-Myelin Oligodendrocyte Glycoprotein (anti-MOG) and anti-Myelin Basic Protein (MBP) which are involved in the demyelinating process. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, GUEDMILLER S.; IERICH, JESSICA C. M.; MORAES, ARIANA S.; SILVA, GISELA B. R. F.; LIU, YANYUN; NETO, LOURIVAL R. DE S.; FARIA, ROBERTO R.; FRANCA, EDUARDO F.; FREITAS, LUIZ C. G.; BRIGGS, JAMES M.; et al. Immobilization and unbinding investigation of the antigen -antibody complex using theoretical and experimental techniques. JOURNAL OF MOLECULAR GRAPHICS & MODELLING, v. 86, p. 219-227, JAN 2019. (12/50839-4, 15/05283-6, 13/09746-5, 14/21530-0, 14/12082-4, 14/26369-3, 15/06847-0)

GARCIA, PAMELA SOTO; BRUM, DORALINA GUIMARAES; OLIVEIRA JR, OSVALDO N.; HIGA, AKEMI MARTINS; MAGALHAES IERICH, JESSICA CRISTIANE; MORAES, ARIANA DE SOUZA; SHIMIZU, FLAVIO MAKOTO; OKUDA-SHINAGAWA, NANCY M.; PERONI, LUIS ANTONIO; DA GAMA, PAULO DINIZ; et al. Nanoimmunosensor based on atomic force spectroscopy to detect anti-myelin basic protein related to early-stage multiple sclerosis. ULTRAMICROSCOPY, v. 211, APR 2020. (14/50869-6, 12/50839-4, 13/14262-7, 14/12082-4, 14/21530-0, 15/06847-0, 15/14360-4)

HIGA, AKEMI M.; MAMBRINI, GIOVANNI P.; IERICH, JESSICA C. M.; GARCIA, PAMELA S.; SCRAMIN, JULIANA A.; PERONI, LUIS A.; OKUDA-SHINAGAWA, NANCY M.; MACHINI, M. TERESA; TRIVINHO-STRIXINO, FRANCISCO; LEITE, FABIO L.. Peptide-Conjugated Silver Nanoparticle for Autoantibody Recognition. Journal of Nanoscience and Nanotechnology, v. 19, n. 12, p. 7564-7573, DEC 2019. (14/12082-4, 10/10813-0, 14/21530-0, 15/06847-0)

HIGA, AKEMI M. M.; MORAES, ARIANA S. S.; SHIMIZU, FLAVIO M. G.; BUENO, RAQUEL G. A.; PERONI, LUIS A. T.; STRIXINO, FRANCISCO T.; SOUSA, NISE A. C. G.; DEFFUNE, ELENICE L.; BOVOLATO, ANA LIVIA C.; OLIVEIRA JR, OSVALDO N.; et al. Anti-aquaporin-4 immunoglobulin G colorimetric detection by silver nanoparticles. Nanomedicine-Nanotechnology Biology and Medicine, v. 41, p. 9-pg., 2022-04-01. (15/05283-6, 14/50869-6, 15/06847-0, 18/22214-6)

MAGALHAES IERICH, JESSICA CRISTIANE; BRUM, DORALINA GUIMARAES; MORAES, ARIANA DE SOUZA; HIGA, AKEMI MARTINS; GARCIA, PAMELA SOTO; MIYAZAKI, CELINA MASSUMI; FERREIRA, MARYSTELA; PERONI, LUIS ANTONIO; DE OLIVEIRA, GUEDMILLER SOUZA; FRANCA, EDUARDO DE FARIA; et al. Antibody-mediated biorecognition of myelin oligodendrocyte glycoprotein: computational evidence of demyelination-related epitopes. SCIENTIFIC REPORTS, v. 9, FEB 14 2019. (13/09746-5, 14/12082-4, 14/15093-7, 14/21530-0, 12/50839-4, 15/05283-6, 14/26369-3, 14/50869-6, 15/06847-0, 16/19387-0)

DE LUCA, VANESSA; HIGA, AKEMI MARTINS; ROMANO, CAMILA MALTA; MAMBRINI, GIOVANNI PIMENTA; PERONI, LUIS ANTONIO; TRIVINHO-STRIXINO, FRANCISCO; LEITE, FABIO LIMA. Cross-reactivity between myelin oligodendrocyte glycoprotein and human endogenous retrovirus W protein: nanotechnological evidence for the potential trigger of multiple sclerosis. Micron, v. 120, p. 66-73, MAY 2019. (14/50869-6, 15/06847-0)

MORAES, ARIANA DE SOUZA; BRUM, DORALINA GUIMARAES; MAGALHAES IERICH, JESSICA CRISTIANE; HIGA, AKEMI MARTINS; JABUR ASSIS, AMANDA STEFANIE; MIYAZAKI, CELINA MASSUMI; SHIMIZU, FLAVIO MAKOTO; PERONI, LUIS ANTONIO; MACHINI, M. TERESA; BARREIRA, AMILTON ANTUNES; et al. A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders. SCIENTIFIC REPORTS, v. 9, NOV 6 2019. (16/19387-0, 14/50869-6, 13/14262-7, 14/15093-7, 12/50839-4, 14/12082-4, 15/05283-6, 15/06847-0, 15/14360-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

HIGA, Akemi Martins. Synthesis and characterization of silver nanoparticles applied to immunoglobulin G detection. 2017. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Medicina Tropical de São Paulo (IMT) São Paulo.