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HIV gp120 V3 loop motif variability in HIV, HCV and HBV coinfected patients: immunological and virological parameters of the progression

Grant number: 15/04991-7
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2015
Effective date (End): July 31, 2016
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Rejane Maria Tommasini Grotto
Grantee:Thais Watanabe
Home Institution: Faculdade de Ciências Agronômicas (FCA). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The HIV/HCV and HIV/HBV coinfection are important public health problem. HIV infection progression is influenced by viral and host factors. The viral factors include the sequence diversity found in the third variable region of the HIVgp120 (V3 loop), which relates to various biological characteristics viral. The tip motif of the V3 loop has a conserved amino acid sequence (GPG) in virus of subtype B, the most frequent in the country. In Brazil, we find the B' variant, with the GWG sequence in the region of the V3 loop tip. Infection by this variant has been associated with a slower progression to AIDS, as well as, the non-syncytium-inducing phenotype (NSI). Studies have shown an association between the NSI variants presence with B' variant in HIV asymptomatic monoinfected patients. Little is known about the influence of this variant in HIV infection evolution in HIV/HCV and/or HIV/HBV coinfected. The goal of this study will be to evaluate the presence of the B' variant of the HIV in patients with HIV/HCV, HIV/HBV or HIV/HCV/HBV infection, to determine the relationship with the progression of HIV infection according clinical, virological and immunological parameters. Correlate the presence of B' variant with the ability to induce syncitia formation these same patients and, to check the relationship of these variants with the HIV infection progression. The viral RNA will be extracted from plasma and used for amplification and sequencing of the HIV gp120 V3 loop region. The sequences will be used for inference of V3 loop phenotype using bioinformatics tools. Data about the HIV infection progression will be assessed using the patient's medical registers.