Quantitative trait loci mapping of traits related to robustness in industrial yeast Pedra-2 (Saccharomyces cerevisiae)

• Grant number: 14/26719-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2015
• End date: July 31, 2019
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Gonçalo Amarante Guimarães Pereira
• Grantee: Alessandro Luis Venega Coradini
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 16/21176-8 - Identification of genes that modify the genotype-phenotype map in Saccharomyces cerevisiae., BE.EP.DR

Abstract

The recognition about environmental consequences arising from use of fossil fuels demand a significantly increase in renewable fuels production,being the bioethanol considered one of the main alternatives. In this context, industrial yeasts have attracted the attention of several research groups and biotechnology companies, because they are considered excellent backgrounds to development of new products. Among the industrial yeasts there is the strain Pedra-2 (PE-2), widely used in Brazilian ethanol production, due its high productivity in industrial environment and ability to tolerate oxidative stress and temperature typically found in the industry. Although the genomic analysis of PE-2 strain has shown the presence of stress-related genes, the genetic basis of their strength are not clearly understood, so a detailed study of QTLs for tolerance to oxidative stress and temperature will contribute significantly to understanding of these mechanisms and consequently for continued development of more productive strains. A large number of derivatives segregant strain from PE-2 (or hybrid PE2 x S288c) will be obtained by flow cytometry using the high-throughput tetrad analysis method. All segregating will be assessed under the stress conditions using standard procedure for high-throughput screening. The segregating identified as tolerant will be sequenced and the QTL will be mapped by bioinformatics and statistics analysis. QTLs potentially related to robustness will be validated using the reciprocal hemizigosidade method.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORADINI, V, ALESSANDRO L.; BEZERRA DE MELLO, FELLIPE DA SILVEIRA; FURLAN, MONIQUE; MANEIRA, CARLA; CARAZZOLLE, MARCELO F.; GUIMARAES PEREIRA, GONCALO AMARANTE; TEIXEIRA, GLEIDSON SILVA. QTL mapping of a Brazilian bioethanol strain links the cell wall protein-encoding gene GAS1 to low pH tolerance in S. cerevisiae. BIOTECHNOLOGY FOR BIOFUELS, v. 14, n. 1, DEC 16 2021. (16/12852-0, 16/02506-7, 15/06677-8, 14/26719-4, 18/03403-2, 13/08293-7)

BEZERRA DE MELLO, FELLIPE DA SILVEIRA; VENEGA CORADINI, ALESSANDRO LUIS; GALVAO TIZEI, PEDRO AUGUSTO; CARAZZOLLE, MARCELO FALSARELLA; GUIMARAES PEREIRA, GONCALO AMARANTE; TEIXEIRA, GLEIDSON SILVA. Static microplate fermentation and automated growth analysis approaches identified a highly-aldehyde resistant Saccharomyces cerevisiae strain. BIOMASS & BIOENERGY, v. 120, p. 49-58, JAN 2019. (14/26719-4, 15/06677-8, 16/02506-7)

BEZERRA DE MELLO, FELLIPE DA SILVEIRA; VENEGA CORADINI, ALESSANDRO LUIS; CARAZZOLLE, MARCELO FALSARELLA; MANEIRA, CARLA; FURLAN, MONIQUE; GUIMARAES PEREIRA, GONCALO AMARANTE; TEIXEIRA, GLEIDSON SILVA. Genetic mapping of a bioethanol yeast strain reveals new targets for hydroxymethylfurfural- and thermotolerance. MICROBIOLOGICAL RESEARCH, v. 263, p. 13-pg., 2022-08-02. (16/12852-0, 15/06677-8, 13/08293-7, 16/02506-7, 14/26719-4, 18/03403-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CORADINI, Alessandro Luis Venega. Mapeamento de QTL de características relacionadas à robustez fermentativa da linhagem industrial Pedra-2 (Saccharomyces cerevisiae). 2019. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Engenharia de Alimentos Campinas, SP.