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In vitro and In situ evaluation of the effect of high-fluoride and arginine-based toothpastes on the control of root caries

Grant number: 15/00817-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2015
Effective date (End): September 30, 2017
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal researcher:Linda Wang
Grantee:Marilia Mattar de Amoêdo Campos Velo
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Associated scholarship(s):16/17247-7 - Root caries managing: The knowledge to prevent, arrest and restore, BE.EP.DR


Root caries is a condition that has become more prevalent with increasing life expectancy and therefore associated with the elderly. Thus, the use of high- fluoride (F) toothpastes (5,000 µg F/g) and toothpaste with standard concentration (1,450 µg F/g) associated with arginine suggest improved effect compared to standard toothpastes (1,000-1,450 µg F/g) on progression of root caries. This study will evaluate the effect of these toothpastes in the control of root caries. Two independent studies, one in vitro and other in situ, will be conducted in which bovine dentin specimens will be selected by surface hardness. In the in vitro study, 36 bovine dentin slabs will be used to assess the inhibition of demineralization. Specimens will be randomized among 3 treatment groups (n=12) and submitted to pH-cycling model, which will last 8 days, remaining daily for 6 h in demineralizing solution and 18 h in remineralizing solution, at 37°C. Slabs will be treated 2x/day, before and after immersion in demineralizing solution. Treatments will be performed with standard toothpaste (1,100 µg F/g-Control group), high-F concentration (5,000 µg F/g) and with conventional concentration (1,450 µg F/g) associated with 1,5 % arginine. For analysis of the variables, percentage of surface hardness loss (%SHL), carious lesion area (”S) and microradiography will be determined in each slab. In the second study, in situ, this will be conducted in triple blind method, during 2 weeks, involving 15 volunteers divided into 3 groups (n=5), who will use a palatine intraoral appliance containing 4 bovine dentin slabs (2 on each side) Of these slabs, 2 will be sound and 2 will be previously induced to demineralization, prior to its insertion on the appliance. Sound slabs will be exposed 8x / day to 20% sucrose, simulating high cariogenic challenge situation. Volunteers will brush the slabs on the appliance 3 x/day with the same toothpaste designated. %SHL in slabs initially sound and surface hardness recovery (%SHR) in artificially carious-like slabs will be analyzed and, zone carious lesion and microradiography will also be conducted to assess the depth of carious lesion.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE AMOEDO CAMPOS VELO, MARILIA MATTAR; SILVERIO AGULHARI, MARIA ANGELICA; RIOS, DANIELA; MAGALHAES, ANA CAROLINA; HONORIO, HEITOR MARQUES; WANG, LINDA. Root caries lesions inhibition and repair using commercial high-fluoride toothpastes with or without tri-calcium phosphate and conventional toothpastes containing or not 1.5% arginine CaCO3: an in situ investigation. CLINICAL ORAL INVESTIGATIONS, v. 24, n. 7 NOV 2019. Web of Science Citations: 0.
DE AMOEDO CAMPOS VELO, MARILIA MATTAR; MAGALHAES, ANA CAROLINA; SHIOTA, AYMEE; HERRERA FARHA, ANA LAURA; GRIZZO, LARISSA TERCILIA; HONORIO, HEITOR MARQUES; WANG, LINDA. Profile of high-fluoride toothpastes combined or not with functionalized tri-calcium phosphate on root dentin caries control: An in vitro study. AMERICAN JOURNAL OF DENTISTRY, v. 31, n. 6, p. 290-296, DEC 2018. Web of Science Citations: 2.
DE AMOEDO CAMPOS VELO, MARILIA MATTAR; HERRERA FARHA, ANA LAURA; DA SILVA SANTOS, PAULO SERGIO; SHIOTA, AYMEE; SANSAVINO, SIMONE ZUQUERATO; SOUZA, ANA TARSILA; HONORIO, HEITOR MARQUES; WANG, LINDA. Radiotherapy alters the composition, structural and mechanical properties of root dentin in vitro. CLINICAL ORAL INVESTIGATIONS, v. 22, n. 8, p. 2871-2878, NOV 2018. Web of Science Citations: 5.

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