Brazilian beef production from feedlots has increased in recent years with market demands on efficiency and meat quality. In addition to system efficiency, as ingredient costs are high, there is concern about the risk of metabolic disorders (ex. Acidosis) caused by high intake of carbohydrates with faster rate of degradation. The use of crude glycerin associated with virginiamycin could enhance lactate utilization by bacteria Megasphaera elsdenii and Selenomonas ruminantium and to reduce the production of lactic acid from Streptococcus bovis and Lactobacilli, which may attenuate the risk of acidosis and increase animal performance. The objective of this study will be to evaluate the effects of glycerin ( 0 or 10% of DM) associated with virgniamicyn ( 0 or 25 ppm of DM) on the digestibility, ruminal fermentation, changes in ruminal microbiota using molecular biotechnology, microbial efficiency, weight gain, blood parameters, growth performance, carcass and meat quality of beef cattle finished in feedlot. Data of animal performance (40 animals) will be analyzed in a completely randomized design using mixed models in a factorial arrangement 2 X 2, crude glycerin and virginiamycin. Ruminal parameters and intestinal flow will be analyzed in a replicate 4x 4 Latin square and the model will include fixed effect of treatment and random effects of steer and period. Data will be submitted to analysis of variance using the PROC MIXED procedure of SAS (version 9.2).
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