Antimicrobial and anti-inflammatory activity of Stryphnodendron barbatiman extract glycolic

• Grant number: 15/17927-5
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2015
• End date: October 31, 2016
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Luciane Dias de Oliveira
• Grantee: Laís Fernanda Ferreira Ferraz
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The use of plant extracts have grown in several areas of health, however, in dentistry its use is still very limited , requiring scientific studies proving its anti- inflammatory and antimicrobial action, for their addition in toothpastes, mouthwashes, irrigation and intracanal medications and other drugs used to treat mouth infections. The objectives of this study are: a) to analyze the in vitro antimicrobial action of Stryphnodendron barbatiman glycolic extract on planktonic culture and biofilms (monotypic) of Candida albicans , Staphylococcus aureus , Enterococcus faecalis , Streptococcus mutans and Pseudomonas aeruginosa; b) to analyze in vitro anti-inflammatory action of S. barbatiman extract in macrophages (RAW 264.7) stimulated by lipopolysaccharide (LPS). For the planktonic form, the microdilution broth method is used, according to the Clinical and Laboratory Standards Institute (CLSI) to determine the minimum inhibitory concentrations (MIC) and minimal microbicidal (CMM). For biofilms standardized suspensions (107 cells/mL) are added to microplate wells and after 48 h at 37°C under agitation will be treated with the extract for 5 min and 24 hs. Will include a control group (physiological solution, NaCl). Then, biofilms must be broken with ultrasonic homogenizer, the suspensions are diluted and plated on Sabouraud's dextrose agar (yeast) or Brain Heart Infusion agar (bacteria). After 48 h, the colony forming units are counted per milliliter (CFU/mL) and the values will be converted to log10. To evaluate the anti-inflammatory action of the extract, macrophages (RAW 264.7) are cultivated in microplates for 24 h, after that, are added to different concentrations of the extract and lipopolysaccharide (LPS) from Escherichia coli (1 ug/ mL / well). After incubation for 24 h (37°C and 5% CO2), the supernatant will be collected to evaluate nitric oxide by the Griess method. The results will be statistically analyzed by ANOVA and Tukey Test (5%).