In vitro and in vivo anti-Candida albicans and anti-Helicobacter pylori activity of extracts of Astronium sp. incorporated or not into nanostructured systems

• Grant number: 13/25432-0
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2014
• End date: March 31, 2016
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Taís Maria Bauab
• Grantee: Taís Maria Bauab
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Brazil has one of the richest biodiversity and stand in a prime position, with around 25% of the world's flora. Additionally, the scientific community has become increasingly interested in medicinal plants and herbal medicines, because they are cheaper than the conventional therapy. Moreover, the irrational use of antimicrobial agents to treat these infections resulted in the emergence of multidrug-resistant strains, and thus increasing failures in drug treatment, which stimulates the use of alternative ways to control infectious diseases. The treatment of chronic diseases is urgent in the national and international context. Among these diseases, the ones that stand out are the diseases that affect the digestive tract (gastric and duodenal ulcers, ulcerative colitis and Crohn's disease), diabetes and cancer. The use of standardized herbal medicines effectively and safely has shown some amazing results to treat these diseases. In order to expand the studies of medicinal plants and contribute to the development of new drugs, an interdisciplinary group proposed the project "Standardized Herbal Medicines for the treatment of chronic diseases" in line with the National Policy of Medicinal Plants and Herbal medicines of the Ministry of Health. Among the plants which will be studied is the genus Astronium (Anacardiaceae) which has many medicinal plants, like A. urundeuva, A. fraxinifolium and A. graveolens. They are popularly used as antipyretics, antidiarrheals, anticancer and antimicrobial, properties attributed to the chemical components, like lignans, flavonoids, triterpenes. In this project, ethanolic extracts from the species A. urundeuva, A. fraxinifolium and A. graveolens will be studied, in order to determine the antimicrobial activity of these plant species using the microplate dilution technique for: Staphylococcus aureus, Escherichia coli, Salmonella sp, Helicobacter pylori and Candida albicans. After that, the extracts will be incorporated in microemulsion systems and re-evaluated for their antimicrobial activity. It is expected that in these formulations, the extracts have their activity increased, ensuring the development of an alternative and effective therapy with lower doses. Furthermore, in vivo assays will be performed in order to determine the anti - vulvovaginal candidiasis, anti-Helicobacter pylori and antiulcerogenic potential of these extracts loaded or not into the proposed systems. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BONIFACIO, BRUNA VIDAL; DOS SANTOS RAMOS, MATHEUS APARECIDO; DA SILVA, PATRICIA BENTO; SILVEIRA NEGRI, KAMILA MARIA; LOPES, ERICA DE OLIVEIRA; DE SOUZA, LEONARDO PEREZ; VILEGAS, WAGNER; PAVAN, FERNANDO ROGERIO; CHORILLI, MARLUS; BAUAB, TAIS MARIA. Nanostructured lipid system as a strategy to improve the anti-Candida albicans activity of Astronium sp.. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 10, p. 5081-5092, 2015. (09/52237-9, 13/25121-5, 13/25432-0)

BONIFACIO, BRUNA VIDAL; DOS SANTOS RAMOS, MATHEUS APARECIDO; DA SILVA, PATRICIA BENTO; SILVEIRA NEGRI, KAMILA MARIA; LOPES, ERICA DE OLIVEIRA; DE SOUZA, LEONARDO PEREZ; VILEGAS, WAGNER; PAVAN, FERNANDO ROGERIO; CHORILLI, MARLUS; BAUAB, TAIS MARIA. Nanostructured lipid system as a strategy to improve the anti-Candida albicans activity of Astronium sp.. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 10, p. 12-pg., 2015-01-01. (09/52237-9, 13/25432-0, 13/25121-5)

DOS SANTOS RAMOS, MATHEUS APARECIDO; CALIXTO, GIOVANA; DE TOLEDO, LUCIANI GASPAR; BONIFACIO, BRUNA VIDAL; DOS SANTOS, LOURDES CAMPANER; GOTTARDO DE ALMEIDA, MARGARETE TERESA; CHORILLI, MARLUS; BAUAB, TAIS MARIA. Liquid crystal precursor mucoadhesive system as a strategy to improve the prophylactic action of Syngonanthus nitens (Bong.) Ruhland against infection by Candida krusei. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 10, p. 7455-7466, 2015. (13/25432-0)

DE AMORIM, MARCELO R.; HILARIO, FELIPE; SANO, PAULO T.; BAUAB, TAIS M.; DOS SANTOS, LOURDES C.. Antimicrobial Activity of Paepalanthus planifolius and its Major Components against Selected Human Pathogens. Journal of the Brazilian Chemical Society, v. 29, n. 4, p. 766-774, APR 2018. (13/12564-6, 15/04899-3, 13/25432-0)

HILARIO, FELIPE; POLINARIO, GIULIA; DE AMORIM, MARCELO RODRIGUES; BATISTA, VICTOR DE SOUSA; DO NASCIMENTO JUNIOR, NAILTON MONTEIRO; ARAUJO, ANGELA REGINA; BAUAB, TAIS MARIA; DOS SANTOS, LOURDES CAMPANER. Spirocyclic lactams and curvulinic acid derivatives from the endophytic fungus Curvularia lunata and their antibacterial and antifungal activities. Fitoterapia, v. 141, MAR 2020. (13/25432-0, 15/04899-3, 18/00187-7)

BONIFACIO, BRUNA VIDAL; MACHADO VILA, TAISSA VIEIRA; MASIERO, ISADORA FANTACINI; DA SILVA, PATRICIA BENTO; DA SILVA, ISABEL CRISTIANE; LOPES, ERICA DE OLIVEIRA; DOS SANTOS RAMOS, MATHEUS APARECIDO; DE SOUZA, LEONARDO PEREZ; VILEGAS, WAGNER; PAVAN, FERNANDO ROGERIO; et al. Antifungal Activity of a Hydroethanolic Extract From Astronium urundeuva Leaves Against Candida albicans and Candida glabrata. FRONTIERS IN MICROBIOLOGY, v. 10, NOV 15 2019. (13/25432-0, 09/52237-9, 15/19011-8, 13/25121-5)

DOS SANTOS RAMOS, MATHEUS APARECIDO; DE TOLEDO, LUCIANI GASPAR; FIORAMONTI CALIXTO, GIOVANA MARIA; BONIFACIO, BRUNA VIDAL; DE FREITAS ARAUJO, MARCELO GONZAGA; DOS SANTOS, LOURDES CAMPANER; GOTTARDO DE ALMEIDA, MARGARETE TERESA; CHORILLI, MARLUS; BAUAB, TAIS MARIA. Syngonanthus nitens Bong. (Rhul.)-Loaded Nanostructured System for Vulvovaginal Candidiasis Treatment. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 17, n. 8, AUG 2016. (16/13784-8, 13/25432-0)

FELIPE HILÁRIO; VANESSA M. CHAPLA; ANGELA R. ARAUJO; PAULO T. SANO; TAÍS M. BAUAB; LOURDES C. DOS SANTOS. Antimicrobial Screening of Endophytic Fungi Isolated from the Aerial Parts of Paepalanthus chiquitensis (Eriocaulaceae) Led to the Isolation of Secondary Metabolites Produced by Fusarium fujikuroi. Journal of the Brazilian Chemical Society, v. 28, n. 8, p. 1389-1395, 2017-08-00. (13/12564-6, 13/25432-0, 15/04899-3)

DOS SANTOS RAMOS, MATHEUS APARECIDO; CALIXTO, GIOVANA; DE TOLEDO, LUCIANI GASPAR; BONIFACIO, BRUNA VIDAL; DOS SANTOS, LOURDES CAMPANER; GOTTARDO DE ALMEIDA, MARGARETE TERESA; CHORILLI, MARLUS; BAUAB, TAIS MARIA. Liquid crystal precursor mucoadhesive system as a strategy to improve the prophylactic action of Syngonanthus nitens (Bong.) Ruhland against infection by Candida krusei. INTERNATIONAL JOURNAL OF NANOMEDICINE, v. 10, p. 12-pg., 2015-01-01. (13/25432-0)