| Grant number: | 15/17995-0 |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| Start date: | November 01, 2015 |
| End date: | October 31, 2016 |
| Field of knowledge: | Agronomical Sciences - Food Science and Technology - Food Engineering |
| Principal Investigator: | Luis Alexandre Pedro de Freitas |
| Grantee: | Natália Cirino Belini |
| Host Institution: | Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil |
Abstract The enzyme technology is now one of the most promising fields for the production of highly valued compounds. In this sense, the government promotes actions in order to encourage the use of enzymes in industrial sectors such as food, disinfectants, pharmaceutical, textile, pulp, paper, and also in the treatment of effluents and wastes. Currently, it has been observed a large increase in the proportion of sanitizing formulations containing enzymes in order to replace the sanitizing present on the market, which are composed of caustics, acids, and toxic solvents that harm the environment and cause wear and materials instruments. Thus, this project aims to produce, isolate and dry microbial protease produced by the fungus Aspergillus niger. To achieve this aim, the strain of Aspergillus niger will be kept in PDA. The cultures will be incubated for seven days in an oven at 30 ° C. After the inoculum preparation and determination of the concentration in a Neubauer chamber, the microorganism will grow in a suspended state. The crude extract obtained will be concentrated by tangential ultrafiltration on a 10kDa membrane system to obtain the product extract. Then, the total protein concentration is determined by the Bradford method, for making the standard curve and measurement of enzymatic activity of protease solution obtained, which is submitted to freeze-drying, spray drying with different adjuvants (mannitol, lactose, maltodextrin ). Finally, the results will be analyzed by evaluating the enzymatic activity of the protease when dry and after evaluation of the powder stability in polypropylene microparticles properly sealed. The cost performance benefit of the powder containing the enzyme and the best drying method in which the enzymatic activity was retained will also be assessed. (AU) | |
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