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Evaluation of antimicrobial agents efficacy released by a novel drug delivery system against pathogenic oral biofilms

Grant number: 15/03424-1
Support type:Scholarships in Brazil - Master
Effective date (Start): October 01, 2015
Effective date (End): March 31, 2017
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Marlise Inêz Klein Furlan
Grantee:Guilherme Roncari Rocha
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


The development of efficacious therapies to control oral biofilms is challenging. Many infectious diseases in humans, including those occurring in the mouth, are caused by virulent biofilms. Topical agents used to treat or prevent oral diseases are generally not retained in the mouth for sufficient duration to exert their full therapeutic potential due to rapid clearance by saliva and food ingestion. Thus, novel strategies for biofilm control focused on retention and sustained delivery of active agents at the site where virulent biofilm develops are warranted. Therefore, nanoparticle carriers (NPC) that bind avidly to hydroxyapatite (the main mineral component of teeth), to tooth pellicle and exopolysaccharides (the major components of biofilm's extracellular matrix) were developed (ACS Nano, 2015; DOI: 10.1021/nn507170s). NPC contains pH-responsive elements that facilitate controlled-release of therapeutic agents within acidic environments, which are found in pathogenic oral biofilms. Hydrophobic antimicrobials (anti-biofilm) such as farnesol can be chemically linked to and released from NPC. Topical treatments with NPC linked farnesol decreases the biomass of pathogenic biofilm and the number and severity of carious lesions in an animal model. However, the optimization of NPC is necessary for increased efficiency to eradicate the formation of pathogenic biofilm, and also for binding to distinct dental materials, because failures of restorative/rehabilitation treatments are frequently associated to the occurrence of new caries lesions. Therefore, the aims of this proposal are to evaluate the binding and retention of NPC, as well as the release of anti-biofilm agents to control the formation of pathogenic biofilms on distinct dental materials (vs. hydroxyapatite). (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROCHA, GUILHERME RONCARI; FLOREZ SALAMANCA, ELKIN JAHIR; DE BARROS, ANA LETICIA; VITORINO LOBO, CARMELIA ISABEL; KLEIN, MARLISE INEZ. Effect of tt-farnesol and myricetin on in vitro biofilm formed by Streptococcus mutans and Candida albicans. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, v. 18, FEB 14 2018. Web of Science Citations: 9.

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