Molecular bioindicators of soil microbiome from corn crop under straw effect to N2O gas mitigation

• Grant number: 15/08564-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2015
• End date: May 31, 2018
• Field of knowledge: Agronomical Sciences - Agronomy - Soil Science
• Principal Investigator: Tsai Siu Mui
• Grantee: Caio Augusto Yoshiura
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated scholarship(s): 16/11268-2 - Uncovering microbial greenhouse gas flux mediators of the maize rhizosphere: how does Azospirillum brasiliense mitigate emissions?, BE.EP.DR

Abstract

Brazil occupies a prominent place in maize production, as the third largest producer in the world. However, current papers about corn crop focus mainly on the potential production of plant and its nutrition, little exploring the role of microorganisms in the soil. Therefore, it is fundamentally important to understand the microbiological processes for soil quality maintenance and the mitigation of GHG emissions in this agricultural system with high demand for nitrogen. In this approach, the use of molecular biology allow explore potential microbial balance groups as biomarkers of biological processes. The microarray platform in the taxonomic and functional analysis (GeoChip) has generated results accurately on microorganisms in biogeochemical cycles. About this project, GHG emission fluxes and qPCR obtained data from macrocosm experiment showed straw as a potential attenuator agent of N2O flux. This way, a second experiment was designed with the purpose of elucidating the dynamics of active microbiome around this input by messenger RNA sequencing. Therefore, the development of this project is to generate information on the interaction of urea, diazotrophic inoculant and straw in rhizospheric community of maize in tropical soils; in order to identify molecular bioindicators related to mitigation of N2O. Consequently, we will apply the microarray platform (GeoChip 5.0) to determine the taxonomic and functional aspects of microbial communities, evaluating their participation in biogeochemical cycles and correlate the GHG fluxes under controlled experiment in macrocosm. Moreover, a second experiment will be developed to elucidate the processes of decomposition of organic matter and availability of straw that maintain the dynamics of microbial communities, which could be used to exploration of specific functional genes for lignocellulose decomposition of straw. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRAGA, LUCAS P. P.; YOSHIURA, CAIO A.; BORGES, CLOVIS D.; HORN, MARCUS A.; BROWN, GEORGE G.; DRAKE, HAROLD L.; TSAI, SIU M.. Disentangling the influence of earthworms in sugarcane rhizosphere. SCIENTIFIC REPORTS, v. 6, DEC 15 2016. (13/22845-2, 15/08564-6, 11/51749-6, 15/11120-2)

VENTURINI, ANDRESSA MONTEIRO; NAKAMURA, FERNANDA MANCINI; GONTIJO, JULIA BRANDAO; DA FRANCA, ALINE GIOVANA; YOSHIURA, CAIO AUGUSTO; MANDRO, JESSICA ADRIELE; TSAI, SIU MUI. Robust DNA protocols for tropical soils. HELIYON, v. 6, n. 5, MAY 2020. (15/12282-6, 15/08564-6, 15/13546-7, 18/14974-0, 14/50320-4, 15/23758-1)

YOSHIURA, CAIO AUGUSTO; VENTURINI, ANDRESSA MONTEIRO; PEREZ BRAGA, LUCAS PALMA; DA FRANCA, ALINE GIOVANA; CATANHO PEREIRA DE LYRA, MARIA DO CARMO; TSAI, SIU MUI; RODRIGUES, JORGE LUIZ MAZZA. Responses of Low-Cost Input Combinations on the Microbial Structure of the Maize Rhizosphere for Greenhouse Gas Mitigation and Plant Biomass Production. FRONTIERS IN PLANT SCIENCE, v. 12, JUN 30 2021. (13/22845-2, 15/13546-7, 15/08564-6, 16/11268-2, 14/50320-4, 19/25931-3)

VENTURINI, ANDRESSA M.; DIAS, NAISSA M. S.; GONTIJO, JULIA B.; YOSHIURA, CAIO A.; PAULA, FABIANA S.; MEYER, KYLE M.; NAKAMURA, FERNANDA M.; DA FRANCA, ALINE G.; BORGES, CLOVIS D.; BARLOW, JOS; et al. Increased soil moisture intensifies the impacts of forest-to-pasture conversion on methane emissions and methane-cycling communities in the Eastern Amazon. Environmental Research, v. 212, p. 13-pg., 2022-04-06. (14/50320-4, 15/13546-7, 15/08564-6, 19/25931-3, 17/09616-5, 17/26138-0, 16/11268-2, 17/09643-2, 17/09952-5, 15/12282-6, 18/14974-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

YOSHIURA, Caio Augusto. Molecular bioindicators prospection from maize rhizosphere microbiota to N2O gas mitigation under stover coverage. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Centro de Energia Nuclear na Agricultura (CENA/STB) Piracicaba.