Production of methylated oxindole alkaloid analogues by precursor-directed biosynthesis using in vitro Uncaria guianensis

• Grant number: 15/18978-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2015
• End date: October 31, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Adriana Aparecida Lopes
• Grantee: Bianca Chioca
• Host Institution: Universidade de Ribeirão Preto (UNAERP). Campus Ribeirão Preto. Ribeirão Preto , SP, Brazil
• Associated research grant: 13/07349-9 - Oxindole alkaloids from Uncaria tomentosa: biosynthetic studies and alkaloids analogues obtained by precursor-directed biosynthesis, AP.JP

Abstract

Natural products chemistry represents an alternative to success in drug discovery. This project proposes an approach to diversification of the structure of a natural product; the so-called "precursor-directed biosynthesis"(BDP). The target of this diversification natural product is the pentacyclic oxindole alkaloids (AOP) and tetracyclic oxindole alkaloids (AOT), accumulated in the species U. guianensis. BDP experiment will be employed to generate new AOP and AOT analogues by supplementation of two methylated tryptamine analogues, main indole biosynthetic precursor, in the medium of in vitro culture of U. guianensis.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOPES, ADRIANA A.; CHIOCA, BIANCA; MUSQUIARI, BRUNO; CREVELIN, EDUARDO J.; FRANCA, SUZELEI DE C.; FERNANDES DA SILVA, MARIA FATIMA DAS G.; PEREIRA, ANA MARIA S.. Unnatural spirocyclic oxindole alkaloids biosynthesis in Uncaria guianensis. SCIENTIFIC REPORTS, v. 9, AUG 5 2019. (13/22609-7, 15/18978-2, 13/07349-9)