Development and characterization of an in vitro atopic dermatitis model

• Grant number: 15/23938-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: March 01, 2016
• End date: July 15, 2016
• Field of knowledge: Biological Sciences - Biology
• Principal Investigator: Silvya Stuchi Maria-Engler
• Grantee: Tatiana Do Nascimento Pedrosa
• Supervisor: Yves Poumay
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: University of Namur (UNamur), Belgium
• Associated to the scholarship: 13/12682-9 - Immunosuppressive effects of UV radiation in immunocompetent epidermal equivalent, BP.DR

Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin condition increasingly common, affecting 15-30% of children in industrialized countries and features a large unmet need for effective therapies. AD is considered a disease with TH2 cell type response characterized by over-expression of proinflammatory cytokines such as IL-4, IL-13 and IL-25. Poumay and colleagues showed that changes in the expression of several genes suggest that keratinocytes RHE respond to depletion of cholesterol as monolayers, however, preserved histology and model barrier function indicates there is some additional activation, probably from the immune system. In this context, this work aims to investigate the tissue response to cholesterol depletion followed by Th2 cytokine exposure in an in vitro model of atopic dermatitis. In the future, this model could be used to evaluate candidate drugs or agents aiming at the prevention or treatment of this pathological condition. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEDROSA, TATIANA DO NASCIMENTO; DE VUYST, EVELYNE; MOUND, ABDALLAH; DE ROUVROIT, CATHERINE LAMBERT; MARIA-ENGLER, SILVYA STUCHI; POUMAY, YVES. Methyl-beta-cyclodextrin treatment combined to incubation with interleukin-4 reproduces major features of atopic dermatitis in a 3D-culture model. ARCHIVES OF DERMATOLOGICAL RESEARCH, v. 309, n. 1, p. 63-69, JAN 2017. (15/23938-0)