Effects of branched chain amino acids in the inflammatory response induced by lipopolysaccharide (LPS) in a macrophage cell line raw 264.7

• Grant number: 15/19801-9
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): January 01, 2016
• Effective date (End): February 28, 2017
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Julio Orlando Tirapegui Toledo
• Grantee: Andrea Bonvini
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Nutritional therapy is critical to the recovery of ill patients, as it avoids the worsening of the disease and prevents malnutrition. However, when the supply of nutrients, particularly amino acid, is less than ideal, there is a commitment from the individual's immune response to increase susceptibility to infections. Recent studies have discovered the interaction of the amino acids and the immune system, obtaining satisfactory results regarding the modulation promoted by them. However, the literature lacks studies that evaluate the immunomodulatory effects of branched chain amino acids, because, due to their different metabolism, dependent skeletal muscle and not of hepatic oxidation, research is concentrated within the sports nutrition. The exploitation of these possible effects is crucial to the progress of treatment for patients in nutritional therapy since it can provide a significant change in clinical outcomes of sickness, as well as offer an improvement in their quality of life. The aim of this work is to study the effects of branched chain amino acids on the functionality and inflammatory response induced by lipopolysaccharide (LPS) in cell line RAW 264.7 macrophages, through the evaluation of the phagocytic activity of cytokine synthesis and protein expression involved in the pathway of NF-ºB. (AU)