Studies of infective processes of Citrus leprosis virus (CiLV-C), with emphasis in the construction of infectious clone and characterization of viral proteins.

• Grant number: 15/10249-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: February 01, 2016
• End date: January 31, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Juliana de Freitas Astúa
• Grantee: Mikhail Oliveira Leastro
• Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 14/08458-9 - Plant viruses transmitted by Brevipalpus mites (Acari: Tenuipalpidae) - BTV: survey, identification, molecular characterization, phylogeny; virus/vector/host relationhip; biology, taxonomy and management of the mite, AP.TEM
• Associated scholarship(s): 17/19898-8 - The involvement of the movement protein (MP) of Citrus leprosis-associated viruses with the natural restraint of systemic viral spread in the plant., BE.EP.PD

Abstract

There is a significant increase in the identification and characterization of plant viruses, the class of phytopathogens that, after the fungi, cause higher yield losses in crops of high economic value around the world, with billions of dollars in losses annually. In particular, citrus leprosis disease is considered one of the major viral diseases of citrus in Brazil. Currently, the state of São Paulo is the largest producer of orange juice and is estimated an annual cost of approximately U$ 80 million to control this disease. Given the importance of the disease, in the past decades many efforts have been made to unravel the pathosystem; however, we still lack a more detailed understanding of the molecular processes involved in the manifestation of leprosis. Considering these aspects, we propose a more detailed study of the molecular factors involved in the infective process. The proposal aims to develop reverse genetic strategies and production of infectious clones for Citrus leprosis virus C (CiLV-C), a +ssRNA virus, and the functional characterization of viral proteins with emphasis on viral movement protein. These strategies are essential to advance on the understanding of viral infective processes and the virus/host/vector interactions, as well as, in the development, in the long term, of efficient strategies to control this pathogen. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LEASTRO, MIKHAIL OLIVEIRA; FREITAS-ASTUA, JULIANA; KITAJIMA, ELLIOT WATANABE; PALLAS, VICENTE; SANCHEZ-NAVARRO, JESUS ANGEL. Dichorhaviruses Movement Protein and Nucleoprotein Form a Protein Complex That May Be Required for Virus Spread and Interacts in vivo With Viral Movement-Related Cilevirus Proteins. FRONTIERS IN MICROBIOLOGY, v. 11, NOV 4 2020. (17/19898-8, 15/10249-1, 17/50222-0)

LEASTRO, MIKHAIL OLIVEIRA; FREITAS-ASTUA, JULIANA; KITAJIMA, ELLIOT WATANABE; PALLAS, VICENTE; SANCHEZ-NAVARRO, JESUS A.. Unravelling the involvement of cilevirus p32 protein in the viral transport. SCIENTIFIC REPORTS, v. 11, n. 1, FEB 3 2021. (15/10249-1, 17/50222-0, 17/19898-8)

LEASTRO, MIKHAIL OLIVEIRA; FREITAS-ASTUA, JULIANA; KITAJIMA, ELLIOT WATANABE; PALLAS, VICENTE; SANCHEZ-NAVARRO, JESUS A.. Membrane Association and Topology of Citrus Leprosis Virus C2 Movement and Capsid Proteins. MICROORGANISMS, v. 9, n. 2, FEB 2021. (15/10249-1, 17/19898-8, 17/50222-0)

LEASTRO, MIKHAIL OLIVEIRA; KITAJIMA, ELLIOT WATANABE; SILVA, MARILIA SANTOS; RESENDE, RENATO OLIVEIRA; FREITAS-ASTUA, JULIANA. Dissecting the Subcellular Localization, Intracellular Trafficking, Interactions, Membrane Association, and Topology of Citrus Leprosis Virus C Proteins. FRONTIERS IN PLANT SCIENCE, v. 9, SEP 11 2018. (15/10249-1, 14/08458-9)

LEASTRO, MIKHAIL OLIVEIRA; FREITAS-ASTUA, JULIANA; KITAJIMA, ELLIOT WATANABE; PALLAS, VICENTE; SANCHEZ-NAVARRO, JESUS ANGEL. Dichorhaviruses Movement Protein and Nucleoprotein Form a Protein Complex That May Be Required for Virus Spread and Interacts in vivo With Viral Movement-Related Cilevirus Proteins. FRONTIERS IN MICROBIOLOGY, v. 11, p. 22-pg., 2020-11-04. (15/10249-1, 17/19898-8, 17/50222-0)

LEASTRO, MIKHAIL OLIVEIRA; ORTEGA CASTRO, DEIBIS YORLENIS; FREITAS-ASTUA, JULIANA; KITAJIMA, ELLIOT WATANABE; PALLAS, VICENTE; SANCHEZ-NAVARRO, JESUS ANGEL. Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity. FRONTIERS IN MICROBIOLOGY, v. 11, JUN 9 2020. (15/10249-1, 17/50222-0, 17/19898-8)