Septic response in Piaractus mesopotamicus infected with Aeromonas hydrophila: pathophysiology and transcriptome

• Grant number: 15/12143-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date until: January 01, 2016
• End date until: March 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Flávio Ruas de Moraes
• Grantee: Gustavo da Silva Claudiano
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The pathogenesis of sepsis involves multiple interrelationships between components of the host and the pathogen responsible for high mortality rates in several animal species. Although it is a major cause of mortality in fish, no shortage of literature on the pathophysiology of sepsis and its modulation mechanisms. Recent results of this lab showed rapid increase in plasma cortisol concentration, inhibition of glucose absorption followed hypocortisolemia and hyperglycemia after intracelomic inoculation Aeromonas hydrophila. T3 and T4 thyroid hormones had decreased their serum concentrations immediately after inoculation of the microorganism. Hormonal changes induced by sepsis triggered changes in metabolic pathways with increased protein and lipid catabolism, utilization of transient anaerobic glycolysis pathway and liver damage. The leucocyte count showed leukopenia and thrombocytopenia after cessation of leukocyte chemotaxis and severe morphological changes. The innate immune system variables showed increased production of reactive oxygen species, the lytic activity of serum and bacterial agglutination, unchanged from the lysozyme concentration. After inoculation of A. hydrophila in pacú were evidenced clinical signs of aeromonose, bacteremia and increased survival of 29%. In order to continue these studies and clarify gaps and controversies of the literature, we intend to new technological approach for the global analysis of genes activated during sepsis and deposit the sequences in GenBank (NCBI) using RNA-seq. These results will elucidate the participation of the different mechanisms of innate-acquired immunity of leukocytes and various chemical mediators involved in the process such as tumor necrosis factor and interleukins pro- and anti-inflammatory, through the identification of differentially expressed RNAs. In addition, we intend to identify resistance genes of this species for possible genetic manipulation as a prophylactic measure in the future. Our objective is to further analyze the profile of the concentrations of sodium, serum potassium and chlorine and the activity of the complement system to answer questions that arose in previous studies. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CLAUDIANO, GUSTAVO S.; ANDRADE, SONIA C. S.; SOUZA, ELAINE C.; YUNIS-AGUINAGA, JEFFERSON; COUTINHO, LUIZ L.; MOREIRA, DEBORA K. T.; GONCALVES, FELIPE C.; MUNDIM, ANTONIO V.; MARZOCCHI-MACHADO, CLENI M.; DE MORAES, FLAVIO R.; et al. Role of neuroendocrine modulation and biochemistry in the sepsis in Piaractus mesopotamicus. General and Comparative Endocrinology, v. 288, MAR 1 2020. (11/20280-2, 14/10231-2, 15/12143-6)