Obesity-induced inflammation, alterations in the microbiota and their effects on the enteric nervous system: study in an experimental model of zebrafish.

• Grant number: 15/21644-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: February 01, 2016
• End date: December 31, 2020
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Niels Olsen Saraiva Câmara
• Grantee: Camila Idelí Morales Fénero
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 12/02270-2 - New cellular, molecular and immunological mechanisms involved in acute and chronic renal injury: the search for new therapeutical approaches, AP.TEM
• Associated scholarship(s): 18/05658-8 - Mechanism of Action of a Bacterial Anti-inflammatory Protein, AimA, BE.EP.DR

Abstract

Diets with high levels of carbohydrates and fats, physical inactivity and stress are inducers of metabolic disorders such as insulin resistance, changes in microbiota and obesity. In the last decade, evidence has linked obesity with a state of chronic low-level inflammation, characterized by a release of cytokines by adipose tissue with a predominantly mononuclear inflammatory infiltrate. The microbiota has also been linked to obesity and metabolic changes, influencing weight gain, insulin sensitivity and the inflammatory state, both in the intestine and other organs. Intestinal functions as motility, secretion of hormones, blood flow modulation and nutrient uptake are controlled by the enteric nervous system (ENS) and are altered in obesity. However, the morphological and physiological changes in glial cells and enteric neurons as a result of inflammation, are largely unexplored points. Even less is known about the influence of microbiota in these events. The zebrafish is being target in the study of human pathologies and emerges as an excellent model for the in vivo analysis of the nervous system and the intestine due to features such as transparency, large numbers of animals per crossing, rapid development and low maintenance costs. Thus, we hypothesized that changes in the microbiota, induced by the inflammation resulting from obesity are responsible for changes in ENS. Thus, the objective of this work is to study the ENS changes during obesity, correlating them with the inflammatory state and the commensal microbiota, using a model of obesity induced by diet in juvenile fish. We hope to demonstrate that obesity model in juvenile fish is a great experimental alternative, and can give results on disease mechanisms, such as obesity.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FENERO, CAMILA MORALES; PADOVANI, BARBARA NUNES; DO AMARAL, MARIANA ABRANTES; BOTTURA DE BARROS, GUILHERME JOSE; XAVIER DE OLIVEIRA, IZABELLA KARINA; HIYANE, MEIRE IOSHIE; SARAIVA CAMARA, NIELS OLSEN. Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, n. 171, MAY 2021. (17/05687-5, 15/21644-9, 18/20722-4, 17/05264-7)

FENERO, CAMILA MORALES; PADOVANI, BARBARA NUNES; DO AMARAL, MARIANA ABRANTES; BOTTURA DE BARROS, GUILHERME JOSE; XAVIER DE OLIVEIRA, IZABELLA KARINA; HIYANE, MEIRE IOSHIE; SARAIVA CAMARA, NIELS OLSEN. Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, v. N/A, n. 171, p. 24-pg., 2021-05-01. (17/05264-7, 17/05687-5, 15/21644-9, 18/20722-4)

TARGHETTA, VITOR PEDRO; AMARAL, MARIANA ABRANTES; SARAIVA CAMARA, NIELS OLSEN. Through DNA sensors and hidden mitochondrial effects of SARS-CoV-2. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 27, AUG 23 2021. (17/05264-7, 15/21644-9, 20/04408-8, 21/00946-8)

DO AMARAL, MARIANA ABRANTES; PAREDES, LAIS CAVALIERI; PADOVANI, BARBARA NUNES; MENDONCA-GOMES, JULIANA MOREIRA; MONTES, LUAN FAVERO; SARAIVA CAMARA, NIELS OLSEN; FENERO, CAMILA MORALES. Mitochondrial connections with immune system in Zebrafish. FISH AND SHELLFISH IMMUNOLOGY REPORTS, v. 2, p. 11-pg., 2021-12-01. (17/05264-7, 15/21644-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FÉNERO, Camila Idelí Morales. Role of the microbiota in low-grade inflammation induced by a high-fat diet in adult zebrafish thesis Ph. D. thesis in Immunology. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.