| Grant number: | 15/25325-5 |
| Support Opportunities: | Scholarships abroad - Research |
| Start date: | June 29, 2016 |
| End date: | March 01, 2017 |
| Field of knowledge: | Biological Sciences - Zoology - Taxonomy of Recent Groups |
| Principal Investigator: | Ricardo Pinto da Rocha |
| Grantee: | Ricardo Pinto da Rocha |
| Host Investigator: | Gonzalo Giribet |
| Host Institution: | Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| Institution abroad: | Harvard University, Cambridge, United States |
| Associated research grant: | 13/50297-0 - Dimensions US-BIOTA São Paulo: a multidisciplinary framework for biodiversity prediction in the Brazilian Atlantic forest hotspot, AP.BTA.TEM |
Abstract The next-generation sequencing (NGS), generates a huge library of small sequences, rather than a single sequence, which may have many base pairs as in the Sanger method. Perhaps the greatest impact of NGS in biogeography / phylogeography is in its ability to help identify and locate the drivers of evolutionary divergence of species, population expansion and persistence in certain environments. The Gonyleptoidea superfamily has not been studied, both from the standpoint of relationship between high taxonomical groups as well as populations of the same species (phylogeography), using NGS. The Museum of Comparative Zoology (MCZ) was chosen due to its forefront in the use of different techniques of NGS and also by having genomic libraries of arachnids. The opportunity of an internship at BPE MCZ will bring not only great benefits for me, by learning new techniques that are still incipient in Brazil, and for the thematic project by obtaining large amounts of data. Knowledge among clades within Gonyleptoidea is still incipient. Studies with phylogeography of harvestmen, published or in progress, have shown that mitochondrial data has been much more informative than the nuclear studied to date. However, the effort in time and money to obtain new markers is too large. Therefore, the sequencing in NGS can easily overcome these difficulties and bring more consistent results than those based on few markers. (AU) | |
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