Physiological function, chemical composition and ontogeny of Echinoid spherulocytes (Echinodermata).

• Grant number: 15/21460-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2016
• End date: November 25, 2019
• Field of knowledge: Biological Sciences - Physiology - Compared Physiology
• Principal Investigator: Márcio Reis Custódio
• Grantee: Vinicius Queiroz Araújo
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 18/14497-8 - Physiological function and origin of the spherulocytes in the sea urchin Paracentrotus lividus (Echinodermata: Echinoidea), BE.EP.DR

Abstract

The blood is a complex tissue possessing very important functions in animal physiology. In the vertebrates, mainly in mammals, the knowledge is high and much is know about the cells forming this tissue. To the invertebrates, this scenario is much different. In these organisms, the celomic fluid is the correspondent to the vertebrate blood, and the cells in this tissue (the coelomocytes) have related roles. Nevertheless, the specific functions of the coelomic cells are poor know. In echinoderms only a cell type, the phagocyte, has been deeply studied. Other coelomic cells, such as the spherulocytes, has been neglected and very few is know about the real amount of subpopulations, the nature of the cytoplasmic inclusion as well as the origin and physiological function of these cells. Thus, in the present project, the goal is to investigate the physiological function of the echinoderms' spherulocytes using sea urchins (Echinoidea) as model organisms. To assess these functions it will be used urchins and sand-dollars, whose spherulocytes subpopulations will first be isolated, characterized and subsequently the cytoplasmic contents will be identified. From these data, it will be performed the experiments regarding the potential physiological roles of these cells. Finally, we will investigate the possible source of origin to these coelomic cells. To achieve these goals, it will be performed some procedures such as: centrifugation in density gradients, flow cytometry, histology, electron microscopy (transmission and scanning), mass spectrometry and larvae farming.

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

QUEIROZ, VINICIUS; ARIZZA, VINCENZO; VAZZANA, MIRELLA; ROZAS, ENRIQUE E.; CUSTODIO, MARCIO R.. Cytocentrifugation as an additional method to study echinoderm coelomocytes: a comparative approach combining living cells, stained preparations, and energy-dispersive x-ray spectroscopy. Revista de Biología Tropical, v. 69, n. 1, p. 171-184, 2021. (13/50218-2, 18/14497-8, 15/21460-5)

QUEIROZ, VINICIUS; MUXEL, SANDRA M.; INGUGLIA, LUIGI; CHIARAMONTE, MARCO; CUSTODIO, MARCIO R.. Comparative study of coelomocytes from Arbacia lixula and Lythechinus variegatus: Cell characterization and in vivo evidence of the physiological function of vibratile cells. FISH & SHELLFISH IMMUNOLOGY, v. 110, p. 1-9, MAR 2021. (18/14497-8, 15/21460-5)

NOGUEIRA, MARCOS M.; NEVES, ELIZABETH; QUEIROZ, VINICIUS; JOHNSSON, RODRIGO. Does coral morpholoy influence the richness and abundance of associated ophiuroids? An evaluation of differences in habitat structure provided by Mussismilia corals. CAHIERS DE BIOLOGIE MARINE, v. 61, n. 2, p. 219-227, 2020. (18/14497-8, 15/21460-5)

QUEIROZ, VINICIUS; ARIZZA, VINCENZO; VAZZANA, MIRELLA; ROZAS, ENRIQUE E.; CUSTODIO, MARCIO R.. Cytocentrifugation as an additional method to study echinoderm coelomocytes: a comparative approach combining living cells, stained preparations, and energy-dispersive x-ray spectroscopy. Revista de Biología Tropical, v. 69, p. 14-pg., 2021-03-01. (15/21460-5, 18/14497-8, 13/50218-2)

QUEIROZ, VINICIUS; NEVES, ELIZABETH; SALES, LICIA; JOHNSSON, RODRIGO. THE GALL-FORMER SABINELLA TROGLODYTES (CAENOGASTROPODA: EULIMIDAE) AND ITS ASSOCIATION WITH EUCIDARIS TRIBULOIDES (ECHINODERMATA: ECHINOIDEA). JOURNAL OF CONCHOLOGY, v. 42, p. 7-pg., 2017-02-01. (15/21460-5, 13/08425-0)

QUEIROZ, VINICIUS; NEVES, ELIZABETH; SALES, LICIA; JOHNSSON, RODRIGO. THE GALL-FORMER SABINELLA TROGLODYTES (CAENOGASTROPODA: EULIMIDAE) AND ITS ASSOCIATION WITH EUCIDARIS TRIBULOIDES (ECHINODERMATA: ECHINOIDEA). JOURNAL OF CONCHOLOGY, v. 42, n. 5, p. 371-377, FEB 2017. (15/21460-5, 13/08425-0)

CHIARAMONTE, MARCO; ARIZZA, VINCENZO; LA ROSA, SIMONA; QUEIROZ, VINICIUS; MAURO, MANUELA; VAZZANA, MIRELLA; INGUGLIA, LUIGI. Allograft Inflammatory Factor AIF-1: early immune response in the Mediterranean sea urchin Paracentrotus lividus. ZOOLOGY, v. 142, OCT 2020. (15/21460-5, 18/14497-8)

QUEIROZ, VINICIUS; MAURO, MANUELA; ARIZZA, VINCENZO; CUSTODIO, MARCIO R.; VAZZANA, MIRELLA. The use of an integrative approach to identify coelomocytes in three species of the genus Holothuria (Echinodermata). Invertebrate Biology, DEC 2021. (15/21460-5, 18/14497-8)

QUEIROZ, VINICIUS. Opportunity makes the thief-observation of a sublethal predation event on an injured sea urchin. MARINE BIODIVERSITY, v. 48, n. 1, p. 153-154, MAR 2018. (15/21460-5)

LIMA, SILVIO FELIPE B.; QUEIROZ, VINICIUS; OLIVEIRA, GERALDO SEMER P.; CHRISTOFFERSEN, MARTIN LINDSEY; GUIMARAES, CARMEN REGINA P.. Stramonita brasiliensis (Gastropoda: Muricidae) living as inquiline on the shell of Pugilina tupiniquim (Gastropoda: Melongenidae). BULLETIN OF MARINE SCIENCE, v. 92, n. 3, p. 371-376, JUL 2016. (15/21460-5)

SALES, LICIA; SANTOS, HENRIQUE B.; LIMA, SILVIO F. B.; QUEIROZ, VINICIUS. A new symbiont for the sea anemone Homostichanthus duerdeni: the spotted cleaner shrimp Periclimenes yucatanicus (Cnidaria, Anthozoa and Crustacea, Decapoda). SPIXIANA, v. 45, n. 1, p. 3-pg., 2022-11-01. (15/21460-5, 18/14497-8, 13/08425-0)

SALES, LICIA; QUEIROZ, VINICIUS. Sexual dimorphism in the parasitic snail Nanobalcis worsfoldi: a histological and morphometric approach with insights for the family Eulimidae. CANADIAN JOURNAL OF ZOOLOGY, v. 99, n. 11, p. 995-1001, NOV 2021. (15/21460-5, 18/14497-8, 13/08425-0)

SALES, LICIA; QUEIROZ, VINICIUS; LUCENA, RUDA A.; MIGOTO, ALVARO E.. An unprecedented observation of the nudibranch Spurilla braziliana (Gastropoda: Aeolidiidae) as a food resource of the pycnogonid Pigrogromitus timsanus (Arthropoda: Ascorhynchoidea). NAUTILUS, v. 135, n. 1, p. 32-34, MAY 13 2021. (15/21460-5, 18/14497-8)

MAURO, MANUELA; QUEIROZ, VINICIUS; ARIZZA, VINCENZO; CAMPOBELLO, DANIELA; CUSTODIO, MARCIO REIS; CHIARAMONTE, MARCO; VAZZANA, MIRELLA. Humoral responses during wound healing in Holothuria tubulosa (Gmelin, 1788). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 253, APR-MAY 2021. (15/21460-5, 18/14497-8)

QUEIROZ, VINICIUS; ARIZZA, VINCENZO; VAZZANA, MIRELLA; CUSTODIO, MARCIO R.. Comparative evaluation of coelomocytes in Paracentrotus sea urchins: Description of new cell types and insights on spherulocyte maturation and sea urchin physiology. ZOOLOGISCHER ANZEIGER, v. 300, p. 14-pg., 2022-07-14. (18/14497-8, 15/21460-5, 21/10161-8)

QUEIROZ, VINICIUS; SALES, LICIA; NEVES, ELIZABETH; JOHNSSON, RODRIGO. Siderastrea stellata Verrill, 1868 as a new host for the corallivore snail Coralliophila caribaea Abbott, 1958 and some biological aspects of their association. SPIXIANA, v. 44, n. 1, p. 2-pg., 2021-11-01. (13/08425-0, 18/14497-8, 15/21460-5)

QUEIROZ, VINICIUS. An unprecedented association of an encrusting bryozoan on the test of a live sea urchin: epibiotic relationship and physiological responses. MARINE BIODIVERSITY, v. 50, n. 5, p. 7-pg., 2020-09-18. (15/21460-5, 18/14497-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

ARAÚJO, Vinicius Queiroz. Diversity, isolation and physiological function of Echinodermata coelomocytes: use of echinoids as model organisms. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB) São Paulo.