Analysis of dendritic cell and T cell pathway in a murine model of chronic pulmonary allergic inflammation with exposition to cigarette smoke

• Grant number: 15/26048-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• Start date: June 01, 2016
• End date: May 31, 2017
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Milton de Arruda Martins
• Grantee: Thayse Regina Bruggemann
• Supervisor: Bruce David Levy
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Harvard University, Boston, United States
• Associated to the scholarship: 14/12398-1 - Analysis of immunonotulation induced by cigarette smoke in two murine models of chronic allergic pulmonary inflammation, BP.DD

Abstract

Several studies have shown that cigarette smoke interacts with the asthma phenotype and also has the potential to interfere in the physical, chemical and immunological mucosal barrier in the airway. In addition, asthmatics subjects who smoke have more severe symptoms and higher numbers of hospitalizations, but the mechanisms for this interaction remains to be determined. Our objective in this project is to determine how dendritic cell (DC) interactions with T cells in allergic inflammation is influenced by cigarette smoke exposure. Murine bone marrow-derived DCs (BMDCs) will be generated and cultured with cigarette smoke extract (CSE). We will evaluate the effect of CSE on BDMC maturation, activation by ovalbumin (OVA) and migration. In addition, using BMDC-T cell co-culture, we will evaluate the effect of CSE on their interactions and activation of T cells by expression of costimulatory molecules. Cytokines in cultured supernatants will be measured by ELISA and cells will be phenotyped by flow cytometry. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DUVALL, MELODY G.; BRUGGEMANN, THAYSE R.; LEVY, BRUCE D.. Bronchoprotective mechanisms for specialized pro-resolving mediators in the resolution of lung inflammation. MOLECULAR ASPECTS OF MEDICINE, v. 58, p. 44-56, DEC 2017. (15/26048-5)

BRUGGEMANN, THAYSE REGINA; FERNANDES, PAULA; OLIVEIRA, LUANA DE MENDONCA; SATO, MARIA NOTOMI; MARTINS, MLTON DE ARRUDA; ARANTES-COSTA, FERNANDA MAGALHAES. Cigarette Smoke Increases CD8 alpha(+) Dendritic Cells in an Ovalbumin-Induced Airway Inflammation. FRONTIERS IN IMMUNOLOGY, v. 8, JUN 16 2017. (14/12398-1, 15/26048-5, 14/21395-6)