Embryonic stem cells cultivation and differentiation in embryoid bodies

• Grant number: 16/03553-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2016
• End date: March 31, 2017
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Henrique Marques Barbosa de Souza
• Grantee: Grazielle Celeste Maktura
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 12/09602-0 - Characterization of the gene regulatory network controled by Coup-TFII in cardiomyocytes derived from embryonic stem cells, AP.JP

Abstract

One of the mysteries in studying the differentiation of CTE as a model for embryonic development is the phenomenon of generating, in vitro, cells and embryonic tissues without signaling located of extraembryonic tissues, essential for the development of the embryo, and morphogenetic movements of gastrulation that culminate in origin and spatial distribution of the three germ layers; ectoderm, mesoderm and endoderm. This phenomenon has been extensively studied through the spontaneous differentiation of CTE into embryoid bodies, cell aggregates in ball form, when grown in suspension, maintain a dimensional relationship, where different programs of cellular differentiation in embryoid bodies are activated in random regions. Recent results obtained in the first year of implementation of this project revealed that the Coup-TFII gene is expressed in embryoid bodies in a hemispheric pattern, meeting with the notion of random spatial patterning observed in embryoid bodies differentiated. (AU)