Synaptonemal complex organization and meiotic behavior of B chromosomes in Astatotilapia latifasciata

• Grant number: 16/03912-9
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: May 02, 2016
• End date: November 01, 2016
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: César Martins
• Grantee: Natália Bortholazzi Venturelli
• Supervisor: Ricardo Benavente
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: Universität Würzburg, Germany
• Associated to the scholarship: 13/25234-4 - Meiotic behavior of B chromosomes in the cichlid fish Astatotilapia latifasciata, based on synaptonemal complex and immunocytogenetic analyses, BP.DR

Abstract

The eukaryote genome is composed of classical DNA sequences and selfish elements, which do not obey the laws of Mendelian inheritance. Among these sequences are transposons, cytoplasmic factors and B chromosomes. Along the advances in chromosomal studies, B chromosomes are no longer seen as parasitic elements, and recent data have shown these chromosomes may have important biological role. B chromosomes studies have been conducted mostly in cytogenetic level, and latest molecular biology approaches may help understanding B chromosome biology. Meiotic behavior analysis associated with the application of new technologies, such as immunostaining and super resolution microscopy, may contribute to better understand the relevance of this genomic element. This project aims to advance understanding the molecular architecture of the synaptonemal complex (SC) using antibodies against SC protein and super resolution microscopy to clarify the meiotic behavior of B chromosomes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARDOSO, ADAUTO LIMA; VENTURELLI, NATALIA BORTHOLAZZI; DA CRUZ, IRENE; DE SA PATRONI, FABIO MALTA; DE MORAES, DIOGO; DE OLIVEIRA, ROGERIO ANTONIO; BENAVENTE, RICARDO; MARTINS, CESAR. Meiotic behavior, transmission and active genes of B chromosomes in the cichlid Astatotilapia latifasciata: new clues about nature, evolution and maintenance of accessory elements. Molecular Genetics and Genomics, v. 297, n. 4, p. 17-pg., 2022-06-15. (16/03912-9, 15/16661-1, 13/25234-4, 16/07743-7, 17/07484-4, 14/15756-6)