Fungemia by yeasts: characterization, virulence factors and antifungal sensitivity of sample isolated from patients with cancer

• Grant number: 15/23545-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2016
• End date: March 31, 2018
• Field of knowledge: Biological Sciences - Microbiology
• Principal Investigator: Luciana da Silva Ruiz Menezes
• Grantee: Milena Barrocali de Araújo Melo
• Host Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Infections caused by Candida species are a major public health problem in tertiary hospitals all over the world, especially in patients with cancer, in which is associated with high mortality. Thus, for the purpose of helping to change this framework, this study will have the following objectives: 1) determine the frequency of yeast species isolated from clinical specimens in specific tertiary public hospital in the care of cancer patients in São Paulo - SP, 2) verify by molecular methods, the presence of C. dubliniensis among the phenotypes of C. albicans, 3) verify, by molecular methods, the prevalence of C. parapsilosis sensu stricto, C. orthopsilosis, C. metapsilosis, C. glabrata sensu stricto, C. bracarensis and C. nivariensis; 4) determine and compare the virulence factors: production of proteinase, phospholipase and hemolysin enzymes; 5) determine and compare the ability of samples to produce biofilm, 6) evaluate the sensitivity patterns of the isolates front antifungals used in the clinic for through the E-test method.