Stromal alterations in prostate cancer: a study on Syndecan proteoglycans family in two knockout mouse model - PTEN-/- e Rb1-/- Trp53-/-

• Grant number: 15/26175-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2016
• End date: February 28, 2018
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Sérgio Luis Felisbino
• Grantee: Nilton José dos Santos
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Prostate cancer (PCa) is the most common malignancy in men and the second leading cause of male cancer-related deaths in the Western world. Studies emphasize the role of stroma compartiment on initiation and progression of CaP. In an ending project, two knockout mice models, the first one exhibits prostate-specific homozygotic deletion of Pten and the other exhibits prostate-specific homozygotic deletion of p53 and pRb, were used to produce trasncriptomes, by next generation sequencing, of normal ventral, lateral, dorsal and anterior prostatic lobes and tumoral samples at different stages of development and progression. Previous analysis of these transcriptome have reveled alteration in the expression of reactive stroma related-genes, such as increased expression of MMPs, type I and type III collagen alpha chains, tenacin C and in the genes of Syndecan 1-4 heparan sulphate proteoglycans family and their cytoplasmatic binding protein Syntenin 1. The aim of this project is to characterize by immunohistochemistry the tissue localization of the 4 members of Syndecan family proteoglycans and Syntenin 1 in the normal and tumoral prostatic tissue during tumor progression and metastasis. These results will contribute for a better understanding of the role of Syndecan proteoglycan family and their binding proteins on reactive stroma associated to the prostate cancer development, progression and metastasis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTOS, NILTON JOSE; BARQUILHA, CAROLINE NASCIMENTO; BARBOSA, ISABELA CORREA; MACEDO, RODRIGO TAVARES; LIMA, FLAVIO OLIVEIRA; JUSTULIN, LUIS ANTONIO; BARBOSA, GUILHERME OLIVEIRA; CARVALHO, HERNANDES F.; FELISBINO, SERGIO LUIS. Syndecan Family Gene and Protein Expression and Their Prognostic Values for Prostate Cancer. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, n. 16, AUG 2021. (16/09532-3, 19/19644-1, 15/26175-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTOS, Nilton José dos. Expression of syndecan and syntenin-1 in the prostate cancer: study in two models of knockouts mice (Pten-/- and Rb1-/- Tpr53-/-) and human prostate. 2018. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.