Sulfiredoxin expression in two prostate cancer knockout mice and by normal and tumoral prostate cell lines under different culture conditions

• Grant number: 15/26097-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): June 01, 2016
• Effective date (End): December 31, 2016
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Sérgio Luis Felisbino
• Grantee: Caroline Nascimento Barquilha
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Studies have shown the role of oxidative stress in the initiation and progression of CaP. Two models of conditional knockout mice, one with homozygote Pten deletion and other with both p53 e pRb homozygote deletions in the prostatic epithelial cells exclusively, were produced and used to obtain the normal and tumoral mouse prostatic lobe transcriptomes, at different stages of tumor progression, using next generation RNA sequencing. Our analysis of these transcriptomes showed altered expression of oxidative stress genes, such as increase in Gpx2 e Gsto1, and reduction of Sod1, Sod3, Prdx6, in which have been already described to play a role in prostate cancer. However, in the undifferentiated tumors (more advanced and aggressive stage) it was observed increased expression of the sulfiredoxin (Srxn1) gene, for which there is no report in prostate cancer yet. The aim of this project is to describe the participation of Srxn1 enzyme in the development and progression of Cap. Initially, the characterization of tissue expression of Sulfiredoxin in the mouse prostate câncer samples will be done by immunohistochemistry. The levels of sulfiredoxin expression by RWPE-1 and PC3 cell lines, at different culture conditions, will also be described, as well as, cellular viability after Srxn1 knockdown by siRNA. These results will contribute to a better understanding of the Srxn1 role in CaP oxidative stress process and can reveal potentials new therapeutic targets for CaP treatment.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARQUILHA, CAROLINE N.; SANTOS, NILTON J.; MONCAO, CAIO C. D.; BARBOSA, ISABELA C.; LIMA, FLAVIO O.; JUSTULIN, LUIS A.; PERTEGA-GOMES, NELMA; FELISBINO, SERGIO L.. Sulfiredoxin as a Potential Therapeutic Target for Advanced and Metastatic Prostate Cancer. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2020, JAN 20 2020. (16/09532-3, 15/26097-6, 16/25945-6)