Cloning SF1 wild type and mutated G372v and T454M in bicistronic retroviral vector for retrovirus production

• Grant number: 16/03982-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): June 01, 2016
• Effective date (End): November 10, 2018
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Leticia Fröhlich Archangelo
• Grantee: Guilherme Brussolo Bidoia
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 14/01458-3 - Defining the functional role of the splicing factor regulator (KIS) during leukemogenesis using a murine bone marrow transplantion model, AP.JP
• Associated scholarship(s): 17/23589-0 - Determination of global RNA splicing in SF1 overexpressed cells by RNA-seq, BE.EP.IC

Abstract

Mutations of proteins components which form the spliceosome, such as U2AF35, ZRSR2, SRSF2, SF3A1, SF3B1, PRP40B, U2AF65, SRSF1 and SF1, are related with tumorigenesis in myeloid and lymphoid lineages. The SF1 gene encodes a protein that acts in the formation of the splicing complex E/A, recognizing the branch point of the intron in the pre-RNA, allowing the interation with the U2AF65 factor, in addition to recruting the U2 snRNP factor. Mutations of this gene are related not only with hematological malignancies, but also to the colon and testicular tumorigenesis. The objective of this project is to produce retroviral particles for transfection of hematopoietic lineage cells, whose study will be of great importance for understanding the performance of SF1 in vivo.