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Determination of global RNA splicing in SF1 overexpressed cells by RNA-seq

Grant number: 17/23589-0
Support type:Scholarships abroad - Research Internship - Scientific Initiation
Effective date (Start): February 01, 2018
Effective date (End): May 31, 2018
Field of knowledge:Biological Sciences - Genetics
Principal Investigator:Leticia Fröhlich Archangelo
Grantee:Guilherme Brussolo Bidoia
Supervisor abroad: Philipp Greif
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Local de pesquisa : Ludwig Maximilian University of Munich (LMU Munich), Germany  
Associated to the scholarship:16/03982-7 - Cloning SF1 wild type and mutated G372v and T454M in bicistronic retroviral vector for retrovirus production, BP.IC

Abstract

Splicing Factor 1 is located on chromosome 11q13. The protein contains an ULM motif, a coiled-coil and KH-QUA2 domains, followed by a zinc knuckle and a proline rich region. SF1 recognizes the branch point sequence within the intron, interacts with the U2AF subunits and signals the first steps of spliceosome assembly. In the alternative splicing, SF1 selectively regulates a subset of introns. Besides, SF1 plays a role in pre-mRNA retention and transcriptional repression. Recently, somatic mutations within SF1 were reported in patients with myelodysplastic syndromes, de novo acute myeloid leukemia and myeloproliferative neoplasms. Also, preliminary data from our group shows an increased SF1 expression in patients with acute lymphoid leukemia. In order to get further insights into SF1 function and possible role in leukemogenesis, the aim of this project is to identify the splicing and transcriptional targets of SF1 in lymphoid cell line overexpressing this protein.

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