Temporal gene expression profiles of sugarcane infected by orange rust

• Grant number: 15/17935-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2016
• End date: January 31, 2017
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Gabriel Rodrigues Alves Margarido
• Grantee: Fernando Henrique Correr
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

Orange rust of sugarcane is caused by Puccinia kuehnii, a recent foliar pathogen in Brazilian sugarcane fields, but already among the major diseases of the crop. Understanding the behavior of such disease is very important in comprehending its mechanisms of infection. This project aims to assess the transcriptome of the plant-pathogen interaction between sugarcane and P. kuehnii, during the establishment and development of the disease, by RNA sequencing using a next generation sequencing platform. The biological material to be used corresponds to sugarcane leaves from the susceptible cultivar SP89-1115 infected with P. kuehnii, sampled at 0h, 12h, 24h, 48h, 5 days and 12 days after infection; each time point was represented by three biological replicates, except for the initial time (control), for which we obtained two replicates. The expression levels of each transcript, in each of the sampling times, will be employed to detect differentially expressed genes with the R package DESeq2. These results will also allow the clustering of transcripts with similar expression patterns, i.e., increasing, decreasing or constant expression level throughout the infection period. Finally, we will perform an evaluation of the relevant metabolic pathways with BLAST2GO, in order to verify the occurrence of their modulation as the pathogen advances on the sugarcane leaves. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORRER, FERNANDO HENRIQUE; HOSAKA, GUILHERME KENICHI; GOMEZ, SERGIO GREGORIO PEREZ; CIA, MARIANA CICARELLI; VITORELLO, CLAUDIA BARROS MONTEIRO; CAMARGO, LUIS EDUARDO ARANHA; MASSOLA JR, NELSON SIDNEI; CARNEIRO, MONALISA SAMPAIO; MARGARIDO, GABRIEL RODRIGUES ALVES. Time-series expression profiling of sugarcane leaves infected with Puccinia kuehnii reveals an ineffective defense system leading to susceptibility. Plant Cell Reports, v. 39, n. 7, APR 2020. (15/22993-7, 15/17935-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CORRER, Fernando Henrique. Temporal gene expression profiles of sugarcane infected by orange rust. 2017. Master's Dissertation - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.