Characterization of the DEAD-box RNA helicase CC1478 in Caulobacter crescentus and its regulation

• Grant number: 16/06378-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2016
• End date: July 31, 2018
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Marilis Do Valle Marques
• Grantee: Rodolfo Alvarenga Ribeiro
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 14/04046-8 - Regulatory networks of bacterial stress response, AP.TEM

Abstract

DEAD-box RNA helicases are enzymes that have a role in several important biologicalprocesses, such as nucleic acids degradation or stabilization, assembly of riboproteiccomplexes and ribosome biogenesis. Caulobacter crescentus is an oligotrophic bacteriumbelonging to the ±-proteobacteria group, which has four genes encoding RNA helicases of theDEAD-box family. Previous work has shown that one of these enzymes, encoded by CC1478,is a functional RNA helicase, but has an atypical carboxi-terminal tail, probably generated bygenetic alterations conserved in the Alpha-proteobacteria.This work aims to characterize theregulation of the helicase CC1478, determining its transcriptional or post-transcriptionalregulation, and evaluate the phylogenetic information to assess the evolutive origin of thisalteration. The importance of this C-terminal domain for the enzyme function will be assessedby deletion of site-directed mutagenesis. The protein expression in the cell cycle and in stressresponse, and its participation in protein complexes, such as the ribosome or RNAdegradosome will be studied.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ASSIS, NADINE G.; RIBEIRO, RODOLFO A.; DA SILVA, LARISSA G.; VICENTE, ALEXANDRE M.; HUG, ISABELLE; MARQUES, MARILIS V.. Identification of Hfq-binding RNAs in Caulobacter crescentus. RNA BIOLOGY, v. 16, n. 6, MAR 2019. (16/06378-3, 14/13552-4, 14/04046-8, 15/21025-7)

SILVA, LARISSA G.; LORENZETTI, ALAN P. R.; RIBEIRO, RODOLFO A.; ALVES, INGRID R.; LEADEN, LAURA; GALHARDO, RODRIGO S.; KOIDE, TIE; MARQUES, MARILIS V.. OxyR and the hydrogen peroxide stress response in Caulobacter crescentus. Gene, v. 700, p. 70-84, JUN 5 2019. (15/07386-7, 16/06378-3, 15/21038-1, 14/04046-8, 17/03052-2)

LEADEN, LAURA; SILVA, LARISSA G.; RIBEIRO, RODOLFO A.; DOS SANTOS, NAARA M.; LORENZETTI, ALAN P. R.; ALEGRIA, THIAGO G. P.; SCHULZ, MARIANE L.; MEDEIROS, MARISA H. G.; KOIDE, TIE; MARQUES, V, MARILIS. Iron Deficiency Generates Oxidative Stress and Activation of the SOS Response in Caulobacter crescentus. FRONTIERS IN MICROBIOLOGY, v. 9, AUG 28 2018. (14/04046-8, 16/06378-3, 15/07386-7, 17/02127-9, 15/21038-1, 17/03052-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RIBEIRO, Rodolfo Alvarenga. Characterization of the DEAD-box RNA helicase CC1478 in Caulobacter crescentus and its regulation.. 2018. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.