Electroforming giant unilamellar vesicles applied as biomembranes mimetic systems

• Grant number: 16/06424-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2016
• End date: April 30, 2019
• Field of knowledge: Physical Sciences and Mathematics - Physics - Condensed Matter Physics
• Principal Investigator: Carlos José Leopoldo Constantino
• Grantee: Tibebe Lemma
• Host Institution: Faculdade de Ciências e Tecnologia (FCT). Universidade Estadual Paulista (UNESP). Campus de Presidente Prudente. Presidente Prudente , SP, Brazil
• Associated research grant: 13/14262-7 - Nanostructured films from biologically-relevant materials, AP.TEM

Abstract

The project at postdoc level envisage the application of confocal microscopy to analyze the interaction at molecular level between distinct analytes and biological membrane models. The main activities to be conducted are: i) prepare giant unilamellar vesicles (GUVs) of zwiterionic phospholipids and cholesterol using the electroformation method, ii) probe analytes-GUVs interactions by adding different concentrations of the analytes to the external environment surrounding the GUVs, iii) propose molecular mechanisms involved in the analytes-phospholipids interaction, iv) develop new applications using phase contrast, fluorescence and confocal microscopies toward biomembrane mimetic systems (GUVs adhesion on modified substrates, Langmuir-Blodgett, Langmuir-Schaefer, dipping- and spray-Layer-by- Layer films). (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LEMMA, TIBEBE; MARQUES RUIZ, GILIA CRISTINE; OLIVEIRA, JR., OSVALDO N.; CONSTANTINO, CARLOS J. L.. The pesticide picloram affects biomembrane models made with Langmuir monolayers. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 181, p. 953-958, SEP 1 2019. (13/14262-7, 16/06424-5)

LEMMA, TIBEBE; SOUZA, FABIANO DE BARROS; TELLEZ SOTO, CLAUDIO A.; MARTIN, AIRTON A.. An FT-Raman, FT-IR, and Quantum Chemical Investigation of Stanozolol and Oxandrolone. BIOSENSORS-BASEL, v. 8, n. 1, MAR 2018. (16/06424-5)

LEMMA, TIBEBE; MARQUES RUIZ, GILIA CRISTINE; OLIVEIRA, JR., OSVALDO N.; CONSTANTINO, CARLOS J. L.. Disruption of giant unilamellar vesicles mimicking cell membranes induced by the pesticides glyphosate and picloram. Biophysical Chemistry, v. 250, JUL 2019. (13/14262-7, 16/06424-5)

LEMMA, TIBEBE; WANG, JIN; ARSTILA, KAI; HYTONEN, VESA P.; TOPPARI, J. JUSSI. Identifying yeasts using surface enhanced Raman spectroscopy. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 218, p. 299-307, JUL 5 2019. (13/14262-7, 16/06424-5)