Global transcriptome analysis of primary subcutaneous preadipocytes~ normal, human: identification of genes regulated by triiodothyronine and irisin

• Grant number: 16/03242-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2016
• End date: May 31, 2019
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Celia Regina Nogueira
• Grantee: Miriane de Oliveira
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Obesity is the most common metabolic disorder in the world, characterized by excess adipose tissue (AT). The literature thus presents several strategic approaches to deal with obese individuals, including food restriction and physical exercise. Thyroid hormones (TH), principally triiodothyronine (T3), they are fundamental to proliferation control and differentiation of AT. The irisin is a protein synthesized and secreted by skeletal muscle of humans and rats is produced after physical exercise and acting on TA stimulating UCP-1, thus leading to release temperature and thereby decrease the fat. Both T3 as irisin can initiate actions resulting in thermogenesis and energy consumption. This study with use of the human cell culture adipocytes aims to identify, by analyzing the transcriptome based on RNAseq, the genes up or down regulated by the action of T3 and/or irisin. The action of T3 on irisin present in TA will be measured by Western blot (WB). The presence and amount of UCP-1 will be evaluate by WB. The DNA damage will be assessed by quantifying the hydroxydeoxyguanosine-8 by ELISA, besides the evaluation of the difference amount of lipids within cells after the treatments. These results this research provide a comprehensive view of the action of triiodothyronine and irisin in the expression of numerous genes in the TA, thus opening the way to explain the pathophysiology of the disease.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, MIRIANE; MATHIAS, LUCAS SOLLA; RODRIGUES, BRUNA MORETTO; MARIANI, BIANCA GONCALVES; GRACELI, JONES BERNARDES; DE SIBIO, MARIA TERESA; CASTRO OLIMPIO, REGIANE MARQUES; FONTES MORETTO, FERNANDA CRISTINA; DEPRA, IGOR CARVALHO; NOGUEIRA, CELIA REGINA. The roles of triiodothyronine and irisin in improving the lipid profile and directing the browning of human adipose subcutaneous cells. Molecular and Cellular Endocrinology, v. 506, APR 15 2020. (16/03242-3)

DE OLIVEIRA, MIRIANE; DE SIBIO, MARIA TERESA; MATHIAS, LUCAS SOLLA; RODRIGUES, BRUNA MORETTO; SAKALEM, MARNA ELIANA; NOGUEIRA, CELIA REGINA. Irisin modulates genes associated with severe coronavirus disease (COVID-19) outcome in human subcutaneous adipocytes cell culture. Molecular and Cellular Endocrinology, v. 515, p. 9-pg., 2020-09-15. (16/03242-3)

DE OLIVEIRA, MIRIANE; MATHIAS, LUCAS SOLLA; DE SIBIO, MARIA TERESA; NORONHA-MATOS, JOSE BERNARDO; COSTA, MARIA ADELINA; NOGUEIRA, CELIA REGINA; CORREIA-DE-SA, PAULO. Pitfalls and challenges of the purinergic signaling cascade in obesity. Biochemical Pharmacology, v. 182, DEC 2020. (16/03242-3)

MATHIAS, LUCAS SOLLA; HERMAN-DE-SOUSA, CARINA; CURY, SARAH SANTILONI; NOGUEIRA, CELIA REGINA; CORREIA-DE-SA, PAULO; DE OLIVEIRA, MIRIANE. RNA-seq reveals that anti-obesity irisin and triiodothyronine (T3) hormones differentially affect the purinergic signaling transcriptomics in differentiated human adipocytes. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, v. 1868, n. 4, p. 15-pg., 2023-01-19. (16/03242-3, 20/06763-0)