Antifungal and anti-inflammatory activity of Rosmarinus officinalis L. extract glycolic (rosemary)

• Grant number: 15/25338-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2016
• End date: December 31, 2018
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Luciane Dias de Oliveira
• Grantee: Vanessa Marques Meccatti Domiciano
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The potential of biological activities of medicinal plants in the control of microorganisms and biological processes such as inflammation has been widely proven, however, in dentistry the use of plant extracts is still very limited. The objectives of this study are to analyze in vitro: a) antifungal activity of R. officinalis L. (rosemary) glycolic extract on planktonic cultures and biofilms (monotypic) of C. albicans, C. glabrata, C. tropicalis, and C. krusei; b) anti-inflammatory action of R. officinalis extract in macrophages (RAW 264.7) stimulated by lipopolysaccharide (LPS). For the planktonic form, the microdilution broth method is used, according to the Clinical and Laboratory Standards Institute (CLSI) M27-A2 on Candida albicans (ATCC 18804), Candida tropicalis (ATCC 13803), Candida glabrata (ATCC 9030) and Candida krusei (ATCC 6258) to determine the minimum inhibitory concentrations (MIC) and minimal microbicidal (CMM). The most effective concentration will be analyzed on biofilms of Candida spp formed for 48 h in microplate wells. To evaluate the anti-inflammatory action of the extract, mouse macrophages (RAW 264.7) are exposed to the most effective concentrations of the extract in the absence and presence of lipopolysaccharide (LPS) from Escherichia coli. For this purpose, ELISA is used to quantify the production of pro-inflammatory cytokines (IL-1² and TNF-±). The results will be statistically analyzed by ANOVA and Tukey test (p d 0.05).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MECCATTI, VANESSA MARQUES; ALVES FIGUEIREDO-GODOI, LIVIA MARA; PEREIRA, THAIS CRISTINE; NAGAI DE LIMA, PATRICIA MICHELLE; ABU HASNA, AMJAD; SENNA, LAVINIA BARBOSA; MARCUCCI, MARIA CRISTINA; JUNQUEIRA, JULIANA CAMPOS; DE OLIVEIRA, LUCIANE DIAS. The biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model. SCIENTIFIC REPORTS, v. 12, n. 1, p. 8-pg., 2022-09-16. (15/25338-0, 15/08776-3)

MECCATTI, VANESSA M.; DE OLIVEIRA, JONATAS R.; FIGUEIRA, LEANDRO W.; NETTO, AMANDIO A. LAGAREIRO; ZAMARIOLI, LUCAS S.; MARCUCCI, MARIA C.; CAMARGO, SAMIRA E. A.; CARVALHO, CLAUDIO A. T.; DE OLIVEIRA, LUCIANE D.. Rosmarinus officinalis L. (rosemary) extract has antibiofilm effect similar to the antifungal nystatin on Candida samples. Anais da Academia Brasileira de Ciências, v. 93, n. 2, 2021. (15/25338-0, 15/08776-3)