The role of C-type lectin receptors in the infection of newborn monocytes by Zika virus

• Grant number: 16/09764-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2016
• End date: March 31, 2018
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Maria Notomi Sato
• Grantee: Fábio Seiti Yamada Yoshikawa
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Zika Virus (ZIKV) is a flavivirus transmitted by Aedes mosquitoes and causes a primary infection similar to Dengue fever but it has also been associated to induction of fetal microcephaly. C-type Lectin Receptors (CLRs) are involved in the recognition of some flavivirus, promoting endocytosis, antigen capture and inflammatory cytokine production, but their contribution to ZIKV infection remains unclear. Therefore, our project aims to investigate the role of CLRs in the interaction between ZIKV and cord blood monocytes. First, we will assess the profile of inflammatory cytokines (IL-1², TNF-±, IL-6, IL-10, IL-12p70, CCL2 e MIF), the activation of CLR signaling molecules (Syk, Raf-1 e DAP12) and the occurrence of cell death (apoptosis or autophagy). Next, we will investigate the specific contribution of DC-SIGN, Mannose Receptor and CLEC5A over these parameters (viral load and cytokine production) through the silencing of each receptor. Due to the urge to understand the ZIKV infection in the neonatal context, the monocyte-virus relationship may be a prominent starting point to the understanding of this disease. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

YAMADA YOSHIKAWA, FABIO SEITI; PIETROBON, ANNA JULIA; CALVIELLI CASTELO BRANCO, ANNA CLAUDIA; PEREIRA, NATALLI ZANETE; DA SILVA OLIVEIRA, LUANDA MARA; MACHADO, CLARISSE MARTINS; DA SILVA DUARTE, ALBERTO JOSE; SATO, MARIA NOTOMI. Zika Virus Infects Newborn Monocytes Without Triggering a Substantial Cytokine Response. Journal of Infectious Diseases, v. 220, n. 1, p. 32-40, JUL 1 2019. (16/09764-1, 16/16840-6)

YAMADA YOSHIKAWA, FABIO SEITI; SATO, MARIA NOTOMI. What are the implications of silent replication in fetal and newborn monocytes for Zika therapeutic development?. FUTURE VIROLOGY, v. 14, n. 5, p. 3-pg., 2019-05-01. (16/16840-6, 16/09764-1)