Therapeutic l-asp for LLA purification employing alternative bioprocess

• Grant number: 16/01869-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: October 01, 2016
• End date: July 31, 2017
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Priscila Gava Mazzola
• Grantee: Louise Lacalendola Tundisi
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 13/08617-7 - Production of extracellular L-asparaginase: from bioprospecting to the engineering of an antileukemic biopharmaceutical, AP.TEM

Abstract

There is a strong global trend to seek alternative routes for the production of active pharmaceutical ingredients obtained by biotechnological processes. However, in both research and in the pharmaceutical industrial production, Brazil has not only a weak expression in the world's scenario but also is losing its international biopharmaceuticals suppliers that are no longer interested in the market and started discontinuing the production of several of these, especially those related to the onco-hematology treatment. In this context, this project proposes the pre-purification of L-asparaginase, biopharmaceutical widely used to treat leucemia as Acute lymphoid leucemia (ALL) that mainly affects children 3-5 years old and adolescents, increasing its survival from 20% to 90%. This purification will be done, by precipitation with ammonium sulfate and ethanol integrated with the enzyme extraction by aqueous two-phase systems (PEG/polyacrylate system and micellar system using Titon X-114 and buffer). The pre-purification tests will be preceded by stability studies of commercial biomolecule and biosynthesized in the presence of the components used for the precipitation and extraction systems. The use of such systems is broad and can be applied at different stages of the enzyme production process, as in steps: clarification, concentration and purification. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOUISE LACALENDOLA TUNDISI; DIEGO DE FREITAS COÊLHO; ALESSANDRA VALÉRIA DE SOUSA FARIA; ADALBERTO PESSOA JUNIOR; ELIAS BASILE TAMBOURGI; LAURA DE OLIVEIRA NASCIMENTO; EDGAR SILVEIRA; PRISCILA GAVA MAZZOLA. Two-Step Purification of L-Asparaginase from Acrylaway® L. Brazilian Journal of Pharmaceutical Sciences, v. 58, 2022-08-08. (13/08617-7, 16/01869-9)

TUNDISI, LOUISE LACALENDOLA; COELHO, DIEGO DE FREITAS; DE SOUSA FARIA, ALESSANDRA VALERIA; PESSOA JUNIOR, ADALBERTO; TAMBOURGI, ELIAS BASILE; NASCIMENTO, LAURA DE OLIVEIRA; SILVEIRA, EDGAR; MAZZOLA, PRISCILA GAVA. Two-Step Purification of L-Asparaginase from Acrylaway((R)) L. Brazilian Journal of Pharmaceutical Sciences, v. 58, p. 13-pg., 2022-01-01. (16/01869-9, 13/08617-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

TUNDISI, Louise Lacalendola. Therapeutic L-asparaginase for acute lymphoid leukemia, purification employing alternative bioprocess. 2017. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.