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Interaction between skeletal muscle resident macrophages and myogenic cells after exercise-induced muscle damage in the CX3CR1-GFP/+ mouse

Grant number: 16/06817-7
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): September 19, 2016
Effective date (End): September 08, 2017
Field of knowledge:Health Sciences - Physical Education
Principal Investigator:Ronaldo Vagner Thomatieli dos Santos
Grantee:Andre Luis Araujo Minari
Supervisor: Chazaud
Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Research place: Université Claude Bernard Lyon 1, France  
Associated to the scholarship:14/04504-6 - The involvement of acetilcoline and glucocorticoids receptors under NFkB transcription in polarization of muscular macrophages after exercise induced damage, BP.DR


ABSTRACTINTRODUCTION: Macrophages (MÕs) and satellite cells (S.C) are closely related to muscular injury and regeneration. After injury (1 to 2 days) MÕs type 1 (M1) provide the clearance of injured cells by phagocytosis and impair myogenesis. During regeneration (4 to 7 days), phagocytosis is concluded and MÕs are simultaneously converted to MÕs type 2 (M2), which restore the process of myogenesis and enable the development of new structures. Besides, few studies shows that acetylcholine and glucocorticoids was involve in this polarization under modulation of NFKB, were they inhibited NFKB proinflamatory reaction and promote up-regulation of anti-inflammatory substances. Based on this, the aim of this project is observe the interaction between skeletal muscle resident macrophages and myogenic cells after exercise-induced muscle damage in the CX3CR1-GFP/+ mouse. Methods: Appliance of exercise-induced injury protocol by downhill exercise (inclination -16°), 18 bouts of run (5min each), At speedy of 17mts/min and rest interval of 2 min. After the protocol of exercise the quadriceps muscle will be dissect and submitted of the isolation of the subsets of MÕs (M1 and M2) trough cell sorter, account and submit to (co) culture of MÕs in medium with myoblasts cells and identify how these macrophages can influence them to differentiate, also, the gene expression analysis of NFkB, nAchR and Glucocorticoids receptors will be analyze by real time PCR. However, procedures will be evaluated immediately, 24, 48 and 72 hours after exercise. The transgenic modified mice will be allocated in 4 experimental groups (G0, G24, G48 and G72) and one control group (CTRL). Statistical analysis: The data will be presented in mean and standard deviation and the adequate test will be determinate by normality test Kolmogorov-Smirnov, according experimental designer.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ARAUJO MINARI, ANDRE LUIS; AVILA, FELIPE; OYAMA, LILA MISSAE; THOMATIELI-SANTOS, RONALDO VAGNER. Skeletal muscles induce recruitment of Ly6C(+) macrophage subtypes and release inflammatory cytokines 3 days after downhill exercise. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, v. 317, n. 4, p. R597-R605, . (17/26563-2, 16/06817-7)

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