Column switching liquid chromatography coupled to tandem mass spectrometry for the analysis of endocannabinoids in plasma samples

• Grant number: 16/01082-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2016
• End date: July 31, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Maria Eugênia Queiroz Nassur
• Grantee: Israel Donizeti de Souza
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 17/24721-0 - Silver-based ionic liquids for the separation and extraction of light olefins using comprehensive two-dimensional gas chromatography, BE.EP.DR

Abstract

Parkinson's disease (PD) is recognized as the second most common neurodegenerative disease in the population. Currently, the DP has no cure; however, the symptoms can be minimized by treatment with new drugs such as cannabidiol. Anandamide and 2- araquidonoglicerol, are endogenous cannabinoids derived from arachidonic acid and they bind to the CB1 and CB2 receptors, acting as agonists. Recently, animal studies suggest a possible change level of these substances in metabolism of animal used as models of PD. However, such studies involving quantification of these compounds in order to elucidate the biochemical this disease are still at an early stage. In recent years, liquid chromatography coupled to tandem mass spectrometry electrospray ionization (ESI) (LC-MS / MS) has become the reference analytical technique for analyzing various substances in biological fluids. The sample preparation step has been required for the development of chromatographic methods to remove interfering compounds and pre-concentrate analytes, often present at trace levels in biological samples. Current analytical chemistry has been directed to simplification by hyphenation techniques, automation (high-throughput performance) and miniaturization of the analytical systems. In this context, we highlight the column switching liquid chromatography coupled to tandem mass spectrometry (LC-MS / MS in column switching mode). The development of stationary phases for the first dimension (1D) of the analytical system, such as polymerizable ionic liquid (PIL) allow direct introduction of biological fluids in chromatographic systems and conduct a selective pre-concentration of the analytes. The great potential of the PIL as sorbent is attributed to its excellent chemical and physicalstability, great sorption capacity and selectivity, which can be adjusted by changes in the structure of the monomer synthesis. In this study, PIL will be synthesized, characterized by different spectroscopic techniques and chemically immobilized on the inner walls of fused silica capillaries. These capillaries will are evaluated as columns 1D LC-MS / MS system in column switching method for the determination of endocannabinoids in plasma samples of patients with PD disease undergoing therapy cannabidiol.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUZA, ISRAEL D.; HANTAO, LEANDRO W.; QUEIROZ, MARIA EUGENIA C.. Polymeric ionic liquid open tubular capillary column for on-line in-tube SPME coupled with UHPLC-MS/MS to determine endocannabinoids in plasma samples. Analytica Chimica Acta, v. 1045, p. 108-116, JAN 3 2019. (17/02147-0, 16/01082-9, 15/07619-1)

SOUZA, ISRAEL D.; NAN, HE; QUEIROZ, MARIA EUGENIA C.; ANDERSON, JARED L.. Tunable Silver-Containing Stationary Phases for Multidimensional Gas Chromatography. Analytical Chemistry, v. 91, n. 8, p. 4969-4974, APR 16 2019. (16/01082-9, 17/24721-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SOUZA, Israel Donizeti de. Development of new capillaries coated with ionic liquids for in-tube SPME-UHPLC-MS / MS and comprehensive two-dimensional gas chromatography (GC × GC). 2019. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (PCARP/BC) Ribeirão Preto.