Comparison of venom from Bothrops jararaca snake maintained in captivity and newly arrived from nature

• Grant number: 16/09976-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2016
• End date: July 31, 2018
• Field of knowledge: Biological Sciences - Physiology - Compared Physiology
• Principal Investigator: Karen de Morais Zani
• Grantee: Nathália da Costa Galizio
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Snake venoms are the most studied animal toxins. The variability of venom composition was described not only intra- and interspecies but also according to sex, age and geographical distribution of the snakes, and in most studies the diet of snakes was considered as the triggering factor for this variation. The Herpetology Laboratory of the Butantan Institute provides venoms for antivenom production and for scientific research not only for the Institute, but to other institutions as well. Currently, it is observed that the number of snakes donated to the Institute has been steadily decreasing. This fact has stimulated the Herpetology Laboratory, where the snakes are kept, to develop technics to reproduce snakes in captivity and to become self-sufficient in creating these animals. Thus, the snake's colony of Herpetology Laboratory is composed mostly by animals born and/or kept in captivity for a long period of time and fed exclusively with mice and/or rats. Considering that the presence of changes in venom composition of snakes kept in captivity has not yet been investigated, the aim of this study is to evaluate the influence captivity maintenance on composition of B. jararaca snake from Herpetology Laboratory of Butantan Institute. This study involves the biological and biochemical characterization and comparison of venoms from captive and wild snakes.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACHADO BRAGA, JACQUELINE RAMOS; DE MORAIS-ZANI, KAREN; PEREIRA, DIEGO DOS SANTOS; SANT'ANNA, SAVIO STEFANINI; GALIZIO, NATHALIA DA COSTA; TANAKA-AZEVEDO, ANITA MITICO; GOMES VILARINHO, ARIEL RODRIGUES; RODRIGUES, JOSE LUCCA; TEIXEIRA DA ROCHA, MARISA MARIA. Sexual and ontogenetic variation of Bothrops leucurus venom. Toxicon, v. 184, p. 127-135, SEP 2020. (14/11108-0, 18/14724-4, 17/16908-2, 16/09976-9, 18/25786-0)

GALIZIO, NATHALIA DA COSTA; SERINO-SILVA, CAROLINE; STUGINSKI, DANIEL RODRIGUES; ESTIMA ABREU, PATRICIA ANTONIA; SANT'ANNA, SAVIO STEFANINI; GREGO, KATHLEEN FERNANDES; TASHIMA, ALEXANDRE KEIJI; TANAKA-AZEVEDO, ANITA MITICO; DE MORAIS-ZANI, KAREN. Compositional and functional investigation of individual and pooled venoms from long-term captive and recently wild-caught Bothrops jararaca snakes. JOURNAL OF PROTEOMICS, v. 186, p. 56-70, AUG 30 2018. (14/11108-0, 16/09976-9, 17/01890-0, 17/16908-2)

DE MORAIS-ZANI, KAREN; SERINO-SILVA, CAROLINE; GALIZIO, NATHALIA DA COSTA; TASIMA, LIDIA JORGE; PAGOTTO, JOSIAS FALARARO; TEIXEIRA DA ROCHA, MARISA MARIA; MARCELINO, JOSE ROBERTO; SANT'ANNA, SAVIO STEFANINI; TASHIMA, ALEXANDRE KEIJI; TANAKA-AZEVEDO, ANITA MITICO; et al. Does the administration of pilocarpine prior to venom milking influence the composition of Micrurus corallinus venom?. JOURNAL OF PROTEOMICS, v. 174, p. 17-27, MAR 1 2018. (14/11108-0, 16/09976-9, 17/01890-0, 12/19321-9)

LEANDRO NORBERTO DA SILVA-JÚNIOR; LARA DE SOUZA ABREU; CAROLINE FABRI BITTENCOURT RODRIGUES; NATHÁLIA DA COSTA GALIZIO; WESLEI DA SILVA AGUIAR; CAROLINE SERINO-SILVA; VALDOMIRO SOUZA DOS SANTOS; ISABELLA ALVES COSTA; LUIS VICENTE FRANCO OLIVEIRA; SÁVIO STEFANINI SANTANNA; et al. Geographic variation of individual venom profile of Crotalus durissus snakes. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 26, 2020-08-26. (18/25786-0, 14/11108-0, 16/09976-9, 18/14724-4, 17/16908-2, 17/01890-0)

VENANCIO DE LIMA, EDUARDO OLIVEIRA; TASIMA, LIDIA JORGE; HATAKEYAMA, DANIELA MIKI; SERINO-SILVA, CAROLINE; BITTENCOURT RODRIGUES, CAROLINE FABRI; GALIZIO, NATHALIA DA COSTA; CHIARELLI, TASSIA; NISHIDUKA, ERIKA SAYURI; TEIXEIRA DA ROCHA, MARISA MARIA; SANT'ANNA, SAVIO STEFANINI; et al. Snake venom color and L-amino acid oxidase: An evidence of long-term captive Crotalus durissus terrificus venom plasticity. Toxicon, v. 193, p. 73-83, APR 15 2021. (18/25786-0, 14/11108-0, 17/01890-0, 16/09976-9, 17/20106-9, 17/21052-0, 17/16908-2, 18/14724-4)